Analysis of RNA expression in adult Zebrafish skeletal muscle

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Abstract

The zebrafish is an excellent vertebrate model system to investigate skeletal muscle development and disease. During early muscle formation the small size of the developing zebrafish allows for the characterization of gene expression in whole embryos. However, as the zebrafish develops, access to the underlying skeletal muscle is limited, requiring the skeletal muscle to be sectioned for a more detailed examination. Here, we describe a straightforward and effective method to prepare adult zebrafish skeletal muscle sections, preserving muscle morphology, to characterize gene expression in the zebrafish adult skeletal muscle.

Original languageEnglish
Title of host publicationSkeletal Muscle Development
PublisherHumana Press
Pages27-35
Number of pages9
Volume1668
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1668
ISSN (Print)10643745

Keywords

  • Pathology
  • RNA in situ hybridization
  • Skeletal muscle
  • Vibratome sectioning
  • Zebrafish adult skeletal muscle

Cite this

Sztal, T. E., Currie, P. D., & Bryson-Richardson, R. J. (2017). Analysis of RNA expression in adult Zebrafish skeletal muscle. In Skeletal Muscle Development (Vol. 1668, pp. 27-35). (Methods in Molecular Biology; Vol. 1668). Humana Press. https://doi.org/10.1007/978-1-4939-7283-8_3