Analysis of native kidney structures in three dimensions

Kieran M Short; Ian M Smyth

doi:10.1007/978-1-61779-851-1_9

Analysis of native kidney structures in three dimensions

Kieran M Short, Ian M Smyth

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

10 Citations (Scopus)

Abstract

Optical Projection Tomography (OPT) is an imaging technique, which has proven to be ideally suited to the observation and quantification of kidney development in rodents. Unlike confocal microscopy systems, OPT is capable of imaging the organ in toto across a long window of embryonic development at sufficient resolution to capture relative changes in branching dynamics, pelvis development, and nephrogenesis. Here, we describe how to image kidneys by OPT, and initial steps to quantify kidney development from this data.

Original language	English
Title of host publication	Kidney Development: Methods and Protocols
Editors	Odyss? Michos
Place of Publication	USA
Publisher	Humana Press
Pages	95 - 107
Number of pages	13
Edition	1st
ISBN (Print)	9781617798504
DOIs	https://doi.org/10.1007/978-1-61779-851-1_9
Publication status	Published - 2012

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10.1007/978-1-61779-851-1_9

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AB - Optical Projection Tomography (OPT) is an imaging technique, which has proven to be ideally suited to the observation and quantification of kidney development in rodents. Unlike confocal microscopy systems, OPT is capable of imaging the organ in toto across a long window of embryonic development at sufficient resolution to capture relative changes in branching dynamics, pelvis development, and nephrogenesis. Here, we describe how to image kidneys by OPT, and initial steps to quantify kidney development from this data.

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BT - Kidney Development: Methods and Protocols

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ER -

Analysis of native kidney structures in three dimensions

Abstract

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