Analysis of isoniazid-resistant transposon mutants of Mycobacterium smegmatis

Helen Billman-Jacobe, Joan Sloan, Ross L. Coppel

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Abstract

The emergence of multidrug-resistant tuberculosis has renewed interest in the study of drug resistance in mycobacteria with the objective of improved chemotherapy. The genetic basis of isoniazid resistance in a model mycobacterium was studied. Eleven isoniazid-resistant mutants of Mycobacterium smegmatis were created using transposon mutagenesis. Genetic and enzymatic characterisation of the mutants showed that katG, encoding T-catalase, was inactivated. The nucleotide sequence of M. smegmatis katG was determined and the mutation sites mapped demonstrating that both the amino and carboxyl halves of T-catalase are important for enzymatic activity.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalFEMS Microbiology Letters
Volume144
Issue number1
DOIs
Publication statusPublished - 15 Oct 1996

Keywords

  • catalase/peroxidase
  • isoniazid
  • katG
  • mycobacteria

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