Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting

Haiyin Liu; Claire Dumont; Angus P R Johnston; Justine D. Mintern

doi:10.1007/978-1-4939-3606-9_15

Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting

Haiyin Liu, Claire Dumont, Angus P R Johnston, Justine D. Mintern

Drug Delivery Disposition & Dynamics

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

9 Citations (Scopus)

Abstract

Antibody-targeted vaccination aims to efficiently deliver antigen to dendritic cells by targeting specific receptors at the cell surface. The choice of receptor depends on different factors, including their capacity to induce internalization of the delivered antigen/adjuvant cargo. Assays currently used to monitor internalization in dendritic cells have several limitations. We have developed a novel DNA-based probe that allows for simple and robust high-throughput analysis of internalization. Designed for flow cytometry, the probe can also be used for fluorescence microscopy to clearly distinguish internalized from surface-bound material. Here, we describe the steps for modifying material (antibodies, proteins) with the probe, undertaking the assay, and analyzing the data obtained from flow cytometry.

Original language	English
Title of host publication	Dendritic Cell Protocols
Editors	Elodie Segura, Nobuyuki Onai
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	15
Pages	199-209
Number of pages	11
Volume	1423
Edition	3rd
ISBN (Electronic)	9781493936069
ISBN (Print)	9781493936045
DOIs	https://doi.org/10.1007/978-1-4939-3606-9_15
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Springer Nature
Volume	1423
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Antibodies
Binding
Dendritic cell
Endocytosis
Flow cytometry
Fluorescence
Internalization
Receptor
Sensor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-3606-9_15

Cite this

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title = "Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting",

abstract = "Antibody-targeted vaccination aims to efficiently deliver antigen to dendritic cells by targeting specific receptors at the cell surface. The choice of receptor depends on different factors, including their capacity to induce internalization of the delivered antigen/adjuvant cargo. Assays currently used to monitor internalization in dendritic cells have several limitations. We have developed a novel DNA-based probe that allows for simple and robust high-throughput analysis of internalization. Designed for flow cytometry, the probe can also be used for fluorescence microscopy to clearly distinguish internalized from surface-bound material. Here, we describe the steps for modifying material (antibodies, proteins) with the probe, undertaking the assay, and analyzing the data obtained from flow cytometry.",

keywords = "Antibodies, Binding, Dendritic cell, Endocytosis, Flow cytometry, Fluorescence, Internalization, Receptor, Sensor",

author = "Haiyin Liu and Claire Dumont and Johnston, {Angus P R} and Mintern, {Justine D.}",

year = "2016",

doi = "10.1007/978-1-4939-3606-9_15",

language = "English",

isbn = "9781493936045",

volume = "1423",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "199--209",

editor = "Segura, {Elodie } and Onai, {Nobuyuki }",

booktitle = "Dendritic Cell Protocols",

address = "United States of America",

edition = "3rd",

}

Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting. / Liu, Haiyin; Dumont, Claire; Johnston, Angus P R et al.
Dendritic Cell Protocols. ed. / Elodie Segura; Nobuyuki Onai. Vol. 1423 3rd. ed. New York NY USA: Humana Press, 2016. p. 199-209 (Methods in Molecular Biology; Vol. 1423).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting

AU - Liu, Haiyin

AU - Dumont, Claire

AU - Johnston, Angus P R

AU - Mintern, Justine D.

PY - 2016

Y1 - 2016

N2 - Antibody-targeted vaccination aims to efficiently deliver antigen to dendritic cells by targeting specific receptors at the cell surface. The choice of receptor depends on different factors, including their capacity to induce internalization of the delivered antigen/adjuvant cargo. Assays currently used to monitor internalization in dendritic cells have several limitations. We have developed a novel DNA-based probe that allows for simple and robust high-throughput analysis of internalization. Designed for flow cytometry, the probe can also be used for fluorescence microscopy to clearly distinguish internalized from surface-bound material. Here, we describe the steps for modifying material (antibodies, proteins) with the probe, undertaking the assay, and analyzing the data obtained from flow cytometry.

AB - Antibody-targeted vaccination aims to efficiently deliver antigen to dendritic cells by targeting specific receptors at the cell surface. The choice of receptor depends on different factors, including their capacity to induce internalization of the delivered antigen/adjuvant cargo. Assays currently used to monitor internalization in dendritic cells have several limitations. We have developed a novel DNA-based probe that allows for simple and robust high-throughput analysis of internalization. Designed for flow cytometry, the probe can also be used for fluorescence microscopy to clearly distinguish internalized from surface-bound material. Here, we describe the steps for modifying material (antibodies, proteins) with the probe, undertaking the assay, and analyzing the data obtained from flow cytometry.

KW - Antibodies

KW - Binding

KW - Dendritic cell

KW - Endocytosis

KW - Flow cytometry

KW - Fluorescence

KW - Internalization

KW - Receptor

KW - Sensor

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U2 - 10.1007/978-1-4939-3606-9_15

DO - 10.1007/978-1-4939-3606-9_15

M3 - Chapter (Book)

AN - SCOPUS:84990056318

SN - 9781493936045

VL - 1423

T3 - Methods in Molecular Biology

SP - 199

EP - 209

BT - Dendritic Cell Protocols

A2 - Segura, Elodie

A2 - Onai, Nobuyuki

PB - Humana Press

CY - New York NY USA

ER -

Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting

Abstract

Publication series

Keywords

UN SDGs

Access to Document

Other files and links

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Cite this

Analysis of intracellular trafficking of dendritic cell receptors for antigen targeting

Abstract

Publication series

Keywords

UN SDGs

Access to Document

Other files and links

Projects

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Cite this