Analysis of human T cell responses to the group II allergen of Dermatophagoides species: Localization of major antigenic sites

Robyn E. O'Hehir, Adrienne Verhoef, Elisabeth Panagiotopoulou, Sanjay Keswani, John D. Hayball, Wayne R. Thomas, Jonathan R. Lamb

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Abstract

Background: IgE antibodies reactive with the group 11 allergens of Dermatophagoides species (house dust mite [HDM]) are a major component of the allergic immune response in HDM-allergic atopic individuals. Methods: Here we demonstrate, with the use of overlapping synthetic peptides of the group II allergen of Dermatophagoides pteronyssinus (Der p II), that polyclonal T cells isolated from the majority of atopic HDM-allergic individuals and healthy nonatopic control subjects respond to Der p II and that T-cell cpitopes are present in all regions of the protein. Results: From comparison of peptide-specific T-cell proliferation in both groups of individuals, it appears that together peptides 61-86 and 78-104 are the most frequently recognized (16 of 18 individuals). We also observed that nine of the 18 individuals responded to T-cell epitopes in the region 11-50, and with Der p Il-reactive T-cell clones, three distinct' T-cell epitopes were mapped within the sequence 11-35. Also, with the use of T-cell clones, two additional epitopes were identified at residues 81-96 and 91-101. Conclusions: These results suggest that T-cell epitopes located in these regions (11-50 and 61-104) are immunodominant. The value of this information in the potential application of Der n II peptides to desensitize HDM allergic responses is discussed.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume92
Issue number1 PART I
DOIs
Publication statusPublished - 1 Dec 1993
Externally publishedYes

Keywords

  • Dennatophagoides species
  • Densensitization
  • Der p II
  • House dust mite
  • Peptides
  • T cells

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