Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation

Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, Matthew P. McCormack

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1 Citation (Scopus)

Abstract

Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Place of PublicationNew York
PublisherHumana Press
Chapter15
Pages177-184
Number of pages8
ISBN (Electronic)9781493975686
ISBN (Print)9781493945679
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1725
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Acute myeloid leukaemia
  • Chromatin
  • Epigenetics
  • Histone modifications
  • Immunoprecipitation

Cite this

Shields, B. J., Keniry, A., Blewitt, M. E., & McCormack, M. P. (2018). Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation. In B. J. Jenkins (Ed.), Inflammation and Cancer: Methods and Protocols (pp. 177-184). (Methods in Molecular Biology; Vol. 1725). New York : Humana Press. https://doi.org/10.1007/978-1-4939-7568-6_15