Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation

Benjamin J. Shields; Andrew Keniry; Marnie E. Blewitt; Matthew P. McCormack

doi:10.1007/978-1-4939-7568-6_15

Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation

Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, Matthew P. McCormack

Australian Centre for Blood Diseases

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

1 Citation (Scopus)

Abstract

Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.

Original language	English
Title of host publication	Inflammation and Cancer
Subtitle of host publication	Methods and Protocols
Editors	Brendan J. Jenkins
Place of Publication	New York
Publisher	Humana Press
Chapter	15
Pages	177-184
Number of pages	8
ISBN (Electronic)	9781493975686
ISBN (Print)	9781493945679
DOIs	https://doi.org/10.1007/978-1-4939-7568-6_15
Publication status	Published - 1 Jan 2018

Publication series

Name	Methods in Molecular Biology
Volume	1725
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Acute myeloid leukaemia
Chromatin
Epigenetics
Histone modifications
Immunoprecipitation

Cite this

Shields, B. J., Keniry, A., Blewitt, M. E., & McCormack, M. P. (2018). Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation. In B. J. Jenkins (Ed.), Inflammation and Cancer: Methods and Protocols (pp. 177-184). (Methods in Molecular Biology; Vol. 1725). New York : Humana Press. https://doi.org/10.1007/978-1-4939-7568-6_15

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Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation. / Shields, Benjamin J.; Keniry, Andrew; Blewitt, Marnie E.; McCormack, Matthew P.

Inflammation and Cancer: Methods and Protocols. ed. / Brendan J. Jenkins. New York : Humana Press, 2018. p. 177-184 (Methods in Molecular Biology; Vol. 1725).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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Access to Document

10.1007/978-1-4939-7568-6_15

Link to publication in Scopus