Abstract
Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
| Original language | English |
|---|---|
| Title of host publication | Inflammation and Cancer |
| Subtitle of host publication | Methods and Protocols |
| Editors | Brendan J. Jenkins |
| Place of Publication | New York |
| Publisher | Humana Press |
| Chapter | 15 |
| Pages | 177-184 |
| Number of pages | 8 |
| ISBN (Electronic) | 9781493975686 |
| ISBN (Print) | 9781493945679 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1725 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Keywords
- Acute myeloid leukaemia
- Chromatin
- Epigenetics
- Histone modifications
- Immunoprecipitation
Cite this
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Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation. / Shields, Benjamin J.; Keniry, Andrew; Blewitt, Marnie E.; McCormack, Matthew P.
Inflammation and Cancer: Methods and Protocols. ed. / Brendan J. Jenkins. New York : Humana Press, 2018. p. 177-184 (Methods in Molecular Biology; Vol. 1725).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
TY - CHAP
T1 - Analysis of histone modifications in acute myeloid leukaemia using chromatin immunoprecipitation
AU - Shields, Benjamin J.
AU - Keniry, Andrew
AU - Blewitt, Marnie E.
AU - McCormack, Matthew P.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
AB - Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
KW - Acute myeloid leukaemia
KW - Chromatin
KW - Epigenetics
KW - Histone modifications
KW - Immunoprecipitation
UR - http://www.scopus.com/inward/record.url?scp=85040664008&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7568-6_15
DO - 10.1007/978-1-4939-7568-6_15
M3 - Chapter (Book)
SN - 9781493945679
T3 - Methods in Molecular Biology
SP - 177
EP - 184
BT - Inflammation and Cancer
A2 - Jenkins, Brendan J.
PB - Humana Press
CY - New York
ER -