Analysis of clonotype distribution and persistence for an influenza virus-specific CD8 + T cell response

Stephen J. Turner, Gabriela Diaz, Richard Cross, Peter C. Doherty

Research output: Contribution to journalArticleResearchpeer-review

119 Citations (Scopus)

Abstract

The spectrum of TCR Vβ usage is compared for primary and recall CD8 + D b PA 224 + T cell responses in mice with influenza pneumonia. Single-cell RT-PCR established that the same clonotypes were present in the lymphoid tissue and in the virus-infected lung. Longitudinal analysis indicated that the memory TCR repertoire reflects the primary response, with no decrease in diversity prior to (or after) secondary challenge. The re-engagement of memory T cells looked to be stochastic in this localized, transient infection. Analysis of clonotypes from the blood, spleen, regional lymph nodes, bone marrow, lung, and liver over a 200 day interval showed no evidence of selective localization or loss. The long-term distribution of memory T cells seemed to be essentially random.

Original languageEnglish
Pages (from-to)549-559
Number of pages11
JournalImmunity
Volume18
Issue number4
DOIs
Publication statusPublished - 1 Apr 2003
Externally publishedYes

Cite this