Analysis of circulating tumor DNA

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2 Citations (Scopus)

Abstract

Circulating tumor DNA (ctDNA) analysis is currently gaining momentum as an innovative methodology for characterizing the tumor genome and monitoring therapeutic efficacy in the multifocal, genetically and spatially heterogeneous plasma cell malignancy, multiple myeloma (MM). Circulating cell-free DNA (cfDNA), which consists of a combination of DNA derived from both tumor and normal cells, is present in extracellular bodily fluids. The presence of ctDNA within this admixture has been demonstrated recently in MM. In this chapter, we describe the routinely utilized methodology for the extraction and longitudinal analysis of specific mutations present in ctDNA derived from peripheral blood plasma of MM patients.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages129-145
Number of pages17
ISBN (Electronic)9781493978656
ISBN (Print)9781493978649
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1792
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell-free DNA
  • Circulating cell-free nucleic acids
  • Circulating tumor DNA
  • Droplet digital PCR
  • Liquid biopsy
  • Multiple myeloma
  • Peripheral blood plasma

Cite this

Mithraprabhu, S., & Spencer, A. (2018). Analysis of circulating tumor DNA. In Methods in Molecular Biology (pp. 129-145). (Methods in Molecular Biology; Vol. 1792). Humana Press. https://doi.org/10.1007/978-1-4939-7865-6_9