Analysis of cellular migration using a two-chamber methodology

Michelle Kouspou, John Price

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

4 Citations (Scopus)

Abstract

Directed cell migration is fundamental to both physiological and pathophysiological processes such as embryogenesis, wound healing, and cancer metastasis. A complex series of events are required for directional cell migration, which is initiated by a migration-promoting or chemotactic stimulus, resulting in cellular polarization and entry into a cyclical pattern of leading edge protrusion, adhesion, and retraction of the trailing edge allowing cell movement. Heat shock proteins such as Hsp27, Hsp90, alphaB-crystallin, as well as heat shock transcription factors, are important players in both physiological and pathophysiological cell migration. A variety of techniques are currently available to assess cell migration, and among the most commonly utilized are those that employ a two-chamber methodology, such as that developed by Stephen Boyden in the early 1960s. Herein, we describe the use of a multiwell microchemotaxis migration assay that has the advantages of being reusable, inexpensive, highly reproducible, and requiring only a small volume of reagents.
Original languageEnglish
Title of host publicationMolecular Chaperones: Methods and Protocols
EditorsStuart K Calderwood, Thomas L Prince
Place of PublicationUSA
PublisherHumana Press
Pages303 - 317
Number of pages15
Edition1st
ISBN (Print)9781617792953
DOIs
Publication statusPublished - 2011

Cite this

Kouspou, M., & Price, J. (2011). Analysis of cellular migration using a two-chamber methodology. In S. K. Calderwood, & T. L. Prince (Eds.), Molecular Chaperones: Methods and Protocols (1st ed., pp. 303 - 317). USA: Humana Press. https://doi.org/10.1007/978-1-61779-295-3_23
Kouspou, Michelle ; Price, John. / Analysis of cellular migration using a two-chamber methodology. Molecular Chaperones: Methods and Protocols. editor / Stuart K Calderwood ; Thomas L Prince. 1st. ed. USA : Humana Press, 2011. pp. 303 - 317
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Kouspou, M & Price, J 2011, Analysis of cellular migration using a two-chamber methodology. in SK Calderwood & TL Prince (eds), Molecular Chaperones: Methods and Protocols. 1st edn, Humana Press, USA, pp. 303 - 317. https://doi.org/10.1007/978-1-61779-295-3_23

Analysis of cellular migration using a two-chamber methodology. / Kouspou, Michelle; Price, John.

Molecular Chaperones: Methods and Protocols. ed. / Stuart K Calderwood; Thomas L Prince. 1st. ed. USA : Humana Press, 2011. p. 303 - 317.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

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AB - Directed cell migration is fundamental to both physiological and pathophysiological processes such as embryogenesis, wound healing, and cancer metastasis. A complex series of events are required for directional cell migration, which is initiated by a migration-promoting or chemotactic stimulus, resulting in cellular polarization and entry into a cyclical pattern of leading edge protrusion, adhesion, and retraction of the trailing edge allowing cell movement. Heat shock proteins such as Hsp27, Hsp90, alphaB-crystallin, as well as heat shock transcription factors, are important players in both physiological and pathophysiological cell migration. A variety of techniques are currently available to assess cell migration, and among the most commonly utilized are those that employ a two-chamber methodology, such as that developed by Stephen Boyden in the early 1960s. Herein, we describe the use of a multiwell microchemotaxis migration assay that has the advantages of being reusable, inexpensive, highly reproducible, and requiring only a small volume of reagents.

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Kouspou M, Price J. Analysis of cellular migration using a two-chamber methodology. In Calderwood SK, Prince TL, editors, Molecular Chaperones: Methods and Protocols. 1st ed. USA: Humana Press. 2011. p. 303 - 317 https://doi.org/10.1007/978-1-61779-295-3_23