Analysis of Antarctic protobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites

L. H. Lee, Y. K. Cheah, A. M. Nurul Syakima, M. S. Shiran, Y. L. Tang, H. P. Lin, K. Hong

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia, Rhizobium, and Staphylococcus. All isolates were studied for capability of producing antimicrobial and antifungal secondary metabolites using high-throughput screening models. Approximately 23 (13/57) and 2% (1/57) of isolates inhibited growth of Candida albicans ATCC 10231T and Staphylococcus aureus ATCC 51650T, respectively. These results indicated that proteobacterium species isolates from Antarctic could serve as potential source of useful bioactive metabolites. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting produced nine clusters and 13 single isolates, with a high D value of 0.9248. RAPD fingerprinting produced six clusters and 13 single isolates, with a relatively low D value of 0.7776. ERIC-PCR analysis proved to have better discrimination capability than RAPD analysis and generated better clustering for all proteobacterium species isolates. We conclude that ERIC-PCR is a robust, reliable and rapid molecular typing method for discriminating different genera of proteobacteria.

Original languageEnglish
Pages (from-to)1627-1641
Number of pages15
JournalGenetics and Molecular Research
Volume11
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • 16S rRNA
  • Diversity
  • ERIC-PCR
  • High-throughput screening
  • Proteobacteria
  • RAPD

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