Analysis of ABCA1 and cholesterol efflux in HIV-infected cells

Nigora Mukhamedova; Beda Brichacek; Christina Darwish; Anastas Popratiloff; Dmitri Sviridov; Michael Bukrinsky

doi:10.1007/978-1-4939-3046-3_19

Analysis of ABCA1 and cholesterol efflux in HIV-infected cells

Nigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, Michael Bukrinsky

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

5 Citations (Scopus)

Abstract

Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press
Pages	281-292
Number of pages	12
Volume	1354
DOIs	https://doi.org/10.1007/978-1-4939-3046-3_19
Publication status	Published - 2016
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1354
ISSN (Print)	10643745

Keywords

ABCA1
Cholesterol efflux
Confocal microscopy
HIV-1
Image analysis
Nef

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-3046-3_19

Cite this

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abstract = "Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.",

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T1 - Analysis of ABCA1 and cholesterol efflux in HIV-infected cells

AU - Mukhamedova, Nigora

AU - Brichacek, Beda

AU - Darwish, Christina

AU - Popratiloff, Anastas

AU - Sviridov, Dmitri

AU - Bukrinsky, Michael

PY - 2016

Y1 - 2016

N2 - Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

AB - Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

KW - ABCA1

KW - Cholesterol efflux

KW - Confocal microscopy

KW - HIV-1

KW - Image analysis

KW - Nef

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DO - 10.1007/978-1-4939-3046-3_19

M3 - Chapter (Book)

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AN - SCOPUS:84952901609

VL - 1354

T3 - Methods in Molecular Biology

SP - 281

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BT - Methods in Molecular Biology

PB - Humana Press

ER -

Analysis of ABCA1 and cholesterol efflux in HIV-infected cells

Abstract

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Keywords

UN SDGs

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