Analysis of ABCA1 and cholesterol efflux in HIV-infected cells

Nigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, Michael Bukrinsky

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

3 Citations (Scopus)

Abstract

Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages281-292
Number of pages12
Volume1354
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)10643745

Keywords

  • ABCA1
  • Cholesterol efflux
  • Confocal microscopy
  • HIV-1
  • Image analysis
  • Nef

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