Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

Trishla Sinha, Arnau Vich Vila, Sanzhima Garmaeva, Soesma A. Jankipersadsing, Floris Imhann, Valerie Collij, Marc Jan Bonder, Xiaofang Jiang, Thomas Gurry, Eric J. Alm, Mauro D’Amato, Rinse K. Weersma, Sicco Scherjon, Cisca Wijmenga, Jingyuan Fu, Alexander Kurilshikov, Alexandra Zhernakova

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.

Original languageEnglish
Pages (from-to)358-366
Number of pages9
JournalGut Microbes
Volume10
Issue number3
DOIs
Publication statusPublished - 2019
Externally publishedYes

Keywords

  • female hormonal factors
  • gut microbiome
  • gut resistome
  • sex differences

Cite this

Sinha, T., Vich Vila, A., Garmaeva, S., Jankipersadsing, S. A., Imhann, F., Collij, V., ... Zhernakova, A. (2019). Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles. Gut Microbes, 10(3), 358-366. https://doi.org/10.1080/19490976.2018.1528822
Sinha, Trishla ; Vich Vila, Arnau ; Garmaeva, Sanzhima ; Jankipersadsing, Soesma A. ; Imhann, Floris ; Collij, Valerie ; Bonder, Marc Jan ; Jiang, Xiaofang ; Gurry, Thomas ; Alm, Eric J. ; D’Amato, Mauro ; Weersma, Rinse K. ; Scherjon, Sicco ; Wijmenga, Cisca ; Fu, Jingyuan ; Kurilshikov, Alexander ; Zhernakova, Alexandra. / Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles. In: Gut Microbes. 2019 ; Vol. 10, No. 3. pp. 358-366.
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abstract = "Several gastrointestinal diseases show a sex imbalance, although the underlying (patho)physiological mechanisms behind this are not well understood. The gut microbiome may be involved in this process, forming a complex interaction with host immune system, sex hormones, medication and other environmental factors. Here we performed sex-specific analyses of fecal microbiota composition in 1135 individuals from a population-based cohort. The overall gut microbiome composition of females and males was significantly different (p = 0.001), with females showing a greater microbial diversity (p = 0.009). After correcting for the effects of intrinsic factors, smoking, diet and medications, female hormonal factors such as the use of oral contraceptives and undergoing an ovariectomy were associated with microbial species and pathways. Females had a higher richness of antibiotic-resistance genes, with the most notable being resistance to the lincosamide nucleotidyltransferase (LNU) gene family. The higher abundance of resistance genes is consistent with the greater prescription of the Macrolide-Lincosamide-Streptogramin classes of antibiotics to females. Furthermore, we observed an increased resistance to aminoglycosides in females with self-reported irritable bowel syndrome. These results throw light upon the effects of common medications that are differentially prescribed between sexes and highlight the importance of sex-specific analysis when studying the gut microbiome and resistome.",
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Sinha, T, Vich Vila, A, Garmaeva, S, Jankipersadsing, SA, Imhann, F, Collij, V, Bonder, MJ, Jiang, X, Gurry, T, Alm, EJ, D’Amato, M, Weersma, RK, Scherjon, S, Wijmenga, C, Fu, J, Kurilshikov, A & Zhernakova, A 2019, 'Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles', Gut Microbes, vol. 10, no. 3, pp. 358-366. https://doi.org/10.1080/19490976.2018.1528822

Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles. / Sinha, Trishla; Vich Vila, Arnau; Garmaeva, Sanzhima; Jankipersadsing, Soesma A.; Imhann, Floris; Collij, Valerie; Bonder, Marc Jan; Jiang, Xiaofang; Gurry, Thomas; Alm, Eric J.; D’Amato, Mauro; Weersma, Rinse K.; Scherjon, Sicco; Wijmenga, Cisca; Fu, Jingyuan; Kurilshikov, Alexander; Zhernakova, Alexandra.

In: Gut Microbes, Vol. 10, No. 3, 2019, p. 358-366.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles

AU - Sinha, Trishla

AU - Vich Vila, Arnau

AU - Garmaeva, Sanzhima

AU - Jankipersadsing, Soesma A.

AU - Imhann, Floris

AU - Collij, Valerie

AU - Bonder, Marc Jan

AU - Jiang, Xiaofang

AU - Gurry, Thomas

AU - Alm, Eric J.

AU - D’Amato, Mauro

AU - Weersma, Rinse K.

AU - Scherjon, Sicco

AU - Wijmenga, Cisca

AU - Fu, Jingyuan

AU - Kurilshikov, Alexander

AU - Zhernakova, Alexandra

PY - 2019

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Sinha T, Vich Vila A, Garmaeva S, Jankipersadsing SA, Imhann F, Collij V et al. Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles. Gut Microbes. 2019;10(3):358-366. https://doi.org/10.1080/19490976.2018.1528822