Analysing tissue and gene function in intestinal organ culture

Helen Elizabeth Abud, Heather Margaret Young, Donald F Newgreen

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

4 Citations (Scopus)

Abstract

The study of growth, differentiation, and migration of different cell types within the developing intestine has been enhanced by the development of mehtods to grow intestinal tissue in organ culture. Here, we describe the innovative method of catenary culture where the tubular architecture of the intesting is maintained and normal cell differentiation occurs. Rapid analysis of gene function can be achieved using low voltage, square wave electroporation to introduce expression constructs into the epithelial cell layer of cultured explants. This whole-organ culture system allows cells, signalling pathways, and gene function to be analysed in intact explants of embryonic gut that are accessible for experimental manipulation and live cell imaging.
Original languageEnglish
Title of host publicationMethods in Molecular Biology, Wnt Signaling
EditorsE Vincan
Place of PublicationUSA
PublisherHumana Press
Pages275 - 286
Number of pages12
Edition1
ISBN (Print)978-1-59745-249-6
Publication statusPublished - 2008

Cite this

Abud, H. E., Young, H. M., & Newgreen, D. F. (2008). Analysing tissue and gene function in intestinal organ culture. In E. Vincan (Ed.), Methods in Molecular Biology, Wnt Signaling (1 ed., pp. 275 - 286). USA: Humana Press.
Abud, Helen Elizabeth ; Young, Heather Margaret ; Newgreen, Donald F. / Analysing tissue and gene function in intestinal organ culture. Methods in Molecular Biology, Wnt Signaling. editor / E Vincan. 1. ed. USA : Humana Press, 2008. pp. 275 - 286
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Abud, HE, Young, HM & Newgreen, DF 2008, Analysing tissue and gene function in intestinal organ culture. in E Vincan (ed.), Methods in Molecular Biology, Wnt Signaling. 1 edn, Humana Press, USA, pp. 275 - 286.

Analysing tissue and gene function in intestinal organ culture. / Abud, Helen Elizabeth; Young, Heather Margaret; Newgreen, Donald F.

Methods in Molecular Biology, Wnt Signaling. ed. / E Vincan. 1. ed. USA : Humana Press, 2008. p. 275 - 286.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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AB - The study of growth, differentiation, and migration of different cell types within the developing intestine has been enhanced by the development of mehtods to grow intestinal tissue in organ culture. Here, we describe the innovative method of catenary culture where the tubular architecture of the intesting is maintained and normal cell differentiation occurs. Rapid analysis of gene function can be achieved using low voltage, square wave electroporation to introduce expression constructs into the epithelial cell layer of cultured explants. This whole-organ culture system allows cells, signalling pathways, and gene function to be analysed in intact explants of embryonic gut that are accessible for experimental manipulation and live cell imaging.

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Abud HE, Young HM, Newgreen DF. Analysing tissue and gene function in intestinal organ culture. In Vincan E, editor, Methods in Molecular Biology, Wnt Signaling. 1 ed. USA: Humana Press. 2008. p. 275 - 286