Abstract
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology, Wnt Signaling |
| Editors | E Vincan |
| Place of Publication | USA |
| Publisher | Humana Press |
| Pages | 275 - 286 |
| Number of pages | 12 |
| Edition | 1 |
| ISBN (Print) | 978-1-59745-249-6 |
| Publication status | Published - 2008 |
Cite this
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Analysing tissue and gene function in intestinal organ culture. / Abud, Helen Elizabeth; Young, Heather Margaret; Newgreen, Donald F.
Methods in Molecular Biology, Wnt Signaling. ed. / E Vincan. 1. ed. USA : Humana Press, 2008. p. 275 - 286.Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review
TY - CHAP
T1 - Analysing tissue and gene function in intestinal organ culture
AU - Abud, Helen Elizabeth
AU - Young, Heather Margaret
AU - Newgreen, Donald F
PY - 2008
Y1 - 2008
N2 - The study of growth, differentiation, and migration of different cell types within the developing intestine has been enhanced by the development of mehtods to grow intestinal tissue in organ culture. Here, we describe the innovative method of catenary culture where the tubular architecture of the intesting is maintained and normal cell differentiation occurs. Rapid analysis of gene function can be achieved using low voltage, square wave electroporation to introduce expression constructs into the epithelial cell layer of cultured explants. This whole-organ culture system allows cells, signalling pathways, and gene function to be analysed in intact explants of embryonic gut that are accessible for experimental manipulation and live cell imaging.
AB - The study of growth, differentiation, and migration of different cell types within the developing intestine has been enhanced by the development of mehtods to grow intestinal tissue in organ culture. Here, we describe the innovative method of catenary culture where the tubular architecture of the intesting is maintained and normal cell differentiation occurs. Rapid analysis of gene function can be achieved using low voltage, square wave electroporation to introduce expression constructs into the epithelial cell layer of cultured explants. This whole-organ culture system allows cells, signalling pathways, and gene function to be analysed in intact explants of embryonic gut that are accessible for experimental manipulation and live cell imaging.
M3 - Chapter (Book)
SN - 978-1-59745-249-6
SP - 275
EP - 286
BT - Methods in Molecular Biology, Wnt Signaling
A2 - Vincan, E
PB - Humana Press
CY - USA
ER -