Abstract
Understanding the behaviour of therapeutic carriers is important in elucidating their mechanism of action and how they are processed inside cells. Herein we examine the intracellular deformation of layer-by-layer assembled polymer capsules using super-resolution structured illumination microscopy (SIM). Spherical- and cylindrical-shaped capsules were studied in three different cell lines, namely HeLa (human epithelial cell line), RAW264.7 (mouse macrophage cell line) and differentiated THP-1 (human monocyte-derived macrophage cell line). We observed that the deformation of capsules was dependent on cell line, but independent of capsule shape. This suggests that the mechanical forces, which induce capsule deformation during cell uptake, vary between cell lines, indicating that the capsules are exposed to higher mechanical forces in HeLa cells, followed by RAW264.7 and then differentiated THP-1 cells. Our study demonstrates the use of super-resolution SIM in analysing intracellular capsule deformation, offering important insights into the cellular processing of drug carriers in cells and providing fundamental knowledge of intracellular mechanobiology. Furthermore, this study may aid in the design of novel drug carriers that are sensitive to deformation for enhanced drug release properties.
| Original language | English |
|---|---|
| Pages (from-to) | 11924-11931 |
| Number of pages | 8 |
| Journal | Nanoscale |
| Volume | 8 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - 21 Jun 2016 |
| Externally published | Yes |
Cite this
}
Analysing intracellular deformation of polymer capsules using structured illumination microscopy. / Chen, Xi; Cui, Jiwei; Sun, Huanli; Müllner, Markus; Yan, Yan; Noi, Ka Fung; Ping, Yuan; Caruso, Frank.
In: Nanoscale, Vol. 8, No. 23, 21.06.2016, p. 11924-11931.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Analysing intracellular deformation of polymer capsules using structured illumination microscopy
AU - Chen, Xi
AU - Cui, Jiwei
AU - Sun, Huanli
AU - Müllner, Markus
AU - Yan, Yan
AU - Noi, Ka Fung
AU - Ping, Yuan
AU - Caruso, Frank
PY - 2016/6/21
Y1 - 2016/6/21
N2 - Understanding the behaviour of therapeutic carriers is important in elucidating their mechanism of action and how they are processed inside cells. Herein we examine the intracellular deformation of layer-by-layer assembled polymer capsules using super-resolution structured illumination microscopy (SIM). Spherical- and cylindrical-shaped capsules were studied in three different cell lines, namely HeLa (human epithelial cell line), RAW264.7 (mouse macrophage cell line) and differentiated THP-1 (human monocyte-derived macrophage cell line). We observed that the deformation of capsules was dependent on cell line, but independent of capsule shape. This suggests that the mechanical forces, which induce capsule deformation during cell uptake, vary between cell lines, indicating that the capsules are exposed to higher mechanical forces in HeLa cells, followed by RAW264.7 and then differentiated THP-1 cells. Our study demonstrates the use of super-resolution SIM in analysing intracellular capsule deformation, offering important insights into the cellular processing of drug carriers in cells and providing fundamental knowledge of intracellular mechanobiology. Furthermore, this study may aid in the design of novel drug carriers that are sensitive to deformation for enhanced drug release properties.
AB - Understanding the behaviour of therapeutic carriers is important in elucidating their mechanism of action and how they are processed inside cells. Herein we examine the intracellular deformation of layer-by-layer assembled polymer capsules using super-resolution structured illumination microscopy (SIM). Spherical- and cylindrical-shaped capsules were studied in three different cell lines, namely HeLa (human epithelial cell line), RAW264.7 (mouse macrophage cell line) and differentiated THP-1 (human monocyte-derived macrophage cell line). We observed that the deformation of capsules was dependent on cell line, but independent of capsule shape. This suggests that the mechanical forces, which induce capsule deformation during cell uptake, vary between cell lines, indicating that the capsules are exposed to higher mechanical forces in HeLa cells, followed by RAW264.7 and then differentiated THP-1 cells. Our study demonstrates the use of super-resolution SIM in analysing intracellular capsule deformation, offering important insights into the cellular processing of drug carriers in cells and providing fundamental knowledge of intracellular mechanobiology. Furthermore, this study may aid in the design of novel drug carriers that are sensitive to deformation for enhanced drug release properties.
UR - http://www.scopus.com/inward/record.url?scp=84974573859&partnerID=8YFLogxK
UR - http://pubs.rsc.org.ezproxy.lib.monash.edu.au/en/content/articlepdf/2016/nr/c6nr02151d
U2 - 10.1039/c6nr02151d
DO - 10.1039/c6nr02151d
M3 - Article
VL - 8
SP - 11924
EP - 11931
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 23
ER -