Abstract
1. The characteristics of the uptake of [3H] L‐noradrenaline from 1 μg pulses injected close arterially to the isolated blood perfused cat spleen are described. 2. The spleen took up 355·0±16·0 ng from a first pulse and 321·1±43·6 ng from a second. 3. Uptake from a second pulse was 254·0±30·3 ng in the presence of the Uptake1 inhibitor desmethylimipramine (DMI) (3·3 × 10−5 M) and the Uptake2 inhibitor 17‐β‐oestradiol (17β0) (1·8 × 10−4 M). 4. Uptake from small 10 ng pulses was also insensitive to DMI and 17β0. 5. Uptake from pulses was abolished by surgical denervation of the spleen, or pre‐treatment with phenoxybenzamine (PBA) (8·8 × 10−5 M). 6. The pulse uptake process was impaired by omission of red cells from the perfusate. 7. Stimulation of the splenic nerves at 3Hz halved the pulse uptake. 8. Uptake from infusion in the presence of DMI and 17β0 occurred only during the first few minutes of the infusion. 9. It is concluded that an uptake process for L‐noradrenaline distinct from Uptakes1 and 2 is present in the spleen and this might be important physiologically.
| Original language | English |
|---|---|
| Pages (from-to) | 193-206 |
| Number of pages | 14 |
| Journal | The Journal of Physiology |
| Volume | 238 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Apr 1974 |
Cite this
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An uptake mechanism for L‐noradrenaline in the cat spleen, associated with the nerves but distinct from uptake1. / Blakeley, A. G H; Powis, G.; Summers, R. J.
In: The Journal of Physiology, Vol. 238, No. 1, 01.04.1974, p. 193-206.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - An uptake mechanism for L‐noradrenaline in the cat spleen, associated with the nerves but distinct from uptake1
AU - Blakeley, A. G H
AU - Powis, G.
AU - Summers, R. J.
PY - 1974/4/1
Y1 - 1974/4/1
N2 - 1. The characteristics of the uptake of [3H] L‐noradrenaline from 1 μg pulses injected close arterially to the isolated blood perfused cat spleen are described. 2. The spleen took up 355·0±16·0 ng from a first pulse and 321·1±43·6 ng from a second. 3. Uptake from a second pulse was 254·0±30·3 ng in the presence of the Uptake1 inhibitor desmethylimipramine (DMI) (3·3 × 10−5 M) and the Uptake2 inhibitor 17‐β‐oestradiol (17β0) (1·8 × 10−4 M). 4. Uptake from small 10 ng pulses was also insensitive to DMI and 17β0. 5. Uptake from pulses was abolished by surgical denervation of the spleen, or pre‐treatment with phenoxybenzamine (PBA) (8·8 × 10−5 M). 6. The pulse uptake process was impaired by omission of red cells from the perfusate. 7. Stimulation of the splenic nerves at 3Hz halved the pulse uptake. 8. Uptake from infusion in the presence of DMI and 17β0 occurred only during the first few minutes of the infusion. 9. It is concluded that an uptake process for L‐noradrenaline distinct from Uptakes1 and 2 is present in the spleen and this might be important physiologically.
AB - 1. The characteristics of the uptake of [3H] L‐noradrenaline from 1 μg pulses injected close arterially to the isolated blood perfused cat spleen are described. 2. The spleen took up 355·0±16·0 ng from a first pulse and 321·1±43·6 ng from a second. 3. Uptake from a second pulse was 254·0±30·3 ng in the presence of the Uptake1 inhibitor desmethylimipramine (DMI) (3·3 × 10−5 M) and the Uptake2 inhibitor 17‐β‐oestradiol (17β0) (1·8 × 10−4 M). 4. Uptake from small 10 ng pulses was also insensitive to DMI and 17β0. 5. Uptake from pulses was abolished by surgical denervation of the spleen, or pre‐treatment with phenoxybenzamine (PBA) (8·8 × 10−5 M). 6. The pulse uptake process was impaired by omission of red cells from the perfusate. 7. Stimulation of the splenic nerves at 3Hz halved the pulse uptake. 8. Uptake from infusion in the presence of DMI and 17β0 occurred only during the first few minutes of the infusion. 9. It is concluded that an uptake process for L‐noradrenaline distinct from Uptakes1 and 2 is present in the spleen and this might be important physiologically.
UR - http://www.scopus.com/inward/record.url?scp=0015984021&partnerID=8YFLogxK
U2 - 10.1113/jphysiol.1974.sp010518
DO - 10.1113/jphysiol.1974.sp010518
M3 - Article
VL - 238
SP - 193
EP - 206
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 1
ER -