An unexpected role for caspase-2 in neuroblastoma

L. Dorstyn, J. Puccini, A. Nikolic, S. Shalini, C. H. Wilson, M. D. Norris, M. Haber, S. Kumar

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Caspase-2 has been implicated in various cellular functions, including cell death by apoptosis, oxidative stress response, maintenance of genomic stability and tumor suppression. The loss of the caspase-2 gene (Casp2) enhances oncogene-mediated tumorigenesis induced by E1A/Ras in athymic nude mice, and also in the El-Myc lymphoma and MMTV/c-neu mammary tumor mouse models. To further investigate the function of caspase-2 in oncogene-mediated tumorigenesis, we extended our studies in the TH-MYCN transgenic mouse model of neuroblastoma. Surprisingly, we found that loss of caspase-2 delayed tumorigenesis in the TH-MYCN neuroblastoma model. In addition, tumors from TH-MYCN/Casp2-/- mice were predominantly thoracic paraspinal tumors and were less vascularized compared with tumors from their TH-MYCN/Casp2 +/+ counterparts. We did not detect any differences in the expression of neuroblastoma-associated genes in TH-MYCN/Casp2-/- tumors, or in the activation of Ras/MAPK signaling pathway that is involved in neuroblastoma progression. Analysis of expression array data from human neuroblastoma samples showed a correlation between low caspase-2 levels and increased survival. However, caspase-2 levels correlated with clinical outcome only in the subset of MYCN-non-amplified human neuroblastoma. These observations indicate that caspase-2 is not a suppressor in MYCN-induced neuroblastoma and suggest a tissue and context-specific role for caspase-2 in tumorigenesis.

Original languageEnglish
Article numbere1383
Number of pages9
JournalCell Death & Disease
Volume5
Issue number8
DOIs
Publication statusPublished - 21 Aug 2014
Externally publishedYes

Keywords

  • apoptosis
  • cell signalling
  • CNS cancer
  • oncogenesis

Cite this