An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer

Muhammad Abdelhamid, Stefania Piantavigna, Alan Maxwell Bond, Bimbil Graham, Leone Spiccia, Lisandra Lorraine Martin, Anthony Peter O'Mullane

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The influence of the membrane active peptides, Tat44–57 (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)6]4− solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat44–57 or melittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers
Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalElectrochemistry Communications
Volume51
DOIs
Publication statusPublished - 2015

Keywords

  • SECM
  • Self-assembled monolayers
  • Electron transfer
  • Peptides

Cite this

Abdelhamid, Muhammad ; Piantavigna, Stefania ; Bond, Alan Maxwell ; Graham, Bimbil ; Spiccia, Leone ; Martin, Lisandra Lorraine ; O'Mullane, Anthony Peter. / An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer. In: Electrochemistry Communications. 2015 ; Vol. 51. pp. 11-14.
@article{742bcf342a0f42b1baf5024003275643,
title = "An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer",
abstract = "The influence of the membrane active peptides, Tat44–57 (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)6]4− solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat44–57 or melittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers",
keywords = "SECM, Self-assembled monolayers, Electron transfer, Peptides",
author = "Muhammad Abdelhamid and Stefania Piantavigna and Bond, {Alan Maxwell} and Bimbil Graham and Leone Spiccia and Martin, {Lisandra Lorraine} and O'Mullane, {Anthony Peter}",
year = "2015",
doi = "10.1016/j.elecom.2014.11.018",
language = "English",
volume = "51",
pages = "11--14",
journal = "Electrochemistry Communications",
issn = "1388-2481",
publisher = "Elsevier",

}

An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer. / Abdelhamid, Muhammad; Piantavigna, Stefania; Bond, Alan Maxwell; Graham, Bimbil; Spiccia, Leone; Martin, Lisandra Lorraine; O'Mullane, Anthony Peter.

In: Electrochemistry Communications, Vol. 51, 2015, p. 11-14.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer

AU - Abdelhamid, Muhammad

AU - Piantavigna, Stefania

AU - Bond, Alan Maxwell

AU - Graham, Bimbil

AU - Spiccia, Leone

AU - Martin, Lisandra Lorraine

AU - O'Mullane, Anthony Peter

PY - 2015

Y1 - 2015

N2 - The influence of the membrane active peptides, Tat44–57 (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)6]4− solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat44–57 or melittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers

AB - The influence of the membrane active peptides, Tat44–57 (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)6]4− solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat44–57 or melittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers

KW - SECM

KW - Self-assembled monolayers

KW - Electron transfer

KW - Peptides

UR - http://goo.gl/h53Eer

U2 - 10.1016/j.elecom.2014.11.018

DO - 10.1016/j.elecom.2014.11.018

M3 - Article

VL - 51

SP - 11

EP - 14

JO - Electrochemistry Communications

JF - Electrochemistry Communications

SN - 1388-2481

ER -