TY - JOUR
T1 - An RNA spiking method demonstrates that 18S rRNA is regulated by progesterone in the mouse uterus
AU - Craythorn, Rebecca Georgette
AU - Girling, Jane Eleanor
AU - Hedger, Mark Peter
AU - Rogers, Peter Adrian Walton
AU - Winnall, Wendy Rachael
PY - 2009
Y1 - 2009
N2 - Identifying suitable housekeeping genes for quantitative RT?PCR in the uterus is problematic, as this tissue undergoes significant
structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of
18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an ?RNA spike? standard into the reverse transcription
reaction. 18S rRNA levels increased by Day 4 of pregnancy and after progesterone administration in ovariectomized mice.We conclude that
18S rRNA is not a suitable housekeeping gene for quantitative RT?PCR analysis in progesterone-responsive tissues, and the RNA spiking method provides a suitable alternative
AB - Identifying suitable housekeeping genes for quantitative RT?PCR in the uterus is problematic, as this tissue undergoes significant
structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of
18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an ?RNA spike? standard into the reverse transcription
reaction. 18S rRNA levels increased by Day 4 of pregnancy and after progesterone administration in ovariectomized mice.We conclude that
18S rRNA is not a suitable housekeeping gene for quantitative RT?PCR analysis in progesterone-responsive tissues, and the RNA spiking method provides a suitable alternative
UR - http://www.ncbi.nlm.nih.gov/pubmed/19602508
UR - https://www.scopus.com/pages/publications/74049087175
U2 - 10.1093/molehr/gap058
DO - 10.1093/molehr/gap058
M3 - Article
SN - 1360-9947
VL - 15
SP - 757
EP - 761
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
IS - 11
ER -