An ovine model for studying the pathophysiology of septic acute kidney injury

Yugeesh R. Lankadeva, Junko Kosaka, Roger G. Evans, Clive N May

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

18 Citations (Scopus)


The development of acute kidney injury (AKI) is both a significant and independent prognostic factor of mortality in patients with sepsis, but its pathophysiology remains unclear. Herein, we describe an ovine model of sepsis evoked by the administration of live Escherichia coli in which there is hypotension, peripheral vasodilatation with a large increase in cardiac output; a similar hyperdynamic state to that commonly reported in humans. Interestingly, in this sheep model of sepsis, despite an increase in global kidney blood flow, there is a progressive reduction in renal function. Although renal hyperperfusion develops, renal tissue hypoxia due to redistribution of intrarenal blood flow may contribute to the pathogenesis of septic AKI. We have, therefore, developed a novel methodology to chronically implant combination probes to monitor intrarenal tissue perfusion and oxygen tension during the development of septic AKI in conscious sheep with hyperdynamic sepsis.

Original languageEnglish
Title of host publicationTraumatic and Ischemic Injury
Subtitle of host publicationMethods and Protocols
EditorsBinu Tharakan
PublisherHumana Press
Number of pages12
ISBN (Electronic)9781493975266
ISBN (Print)9781493975242
Publication statusPublished - 22 Feb 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Acute kidney injury
  • Cardiac output
  • Hypotension
  • Hypoxia
  • Ischemia
  • Renal blood flow
  • Sepsis

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