An orthotopic mouse model of gastric cancer invasion and metastasis

Rita A. Busuttil, David S. Liu, Natasha Di Costanzo, Jan Schröder, Catherine Mitchell, Alex Boussioutas

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Gastric cancer is a leading cause of cancer death worldwide, with advanced stage being correlated to the level of tumour invasion and metastasis. Current research is heavily focused on the identification and development of efficacious therapeutics targeting these fundamental hallmarks of cancer, however there are currently no animal models that mimic the invasive phenotypes observed in humans. To address this we have developed an orthotopic mouse model whereby gastric cancer cell lines are tagged with luciferase and injected into the subserosal layer of the stomach. This allows for the monitoring of primary tumour growth and metastasis in real-time as well as quantitation of the degree of tumour invasion through the stomach wall by immunohistochemistry. We have three models based on the degree of invasion and metastasis that are cell line specific: The AGS cells develop into invasive tumours by 4-weeks with no evidence of metastases, MKN45 cells are moderately metastatic with minimal invasion till week 2 and MKN28 cells are highly metastatic and fully invasive by week 1. These models have utility as a tool for testing the efficacy of anti-tumour, anti-invasive and anti-metastatic therapies in the setting of gastric cancer, which currently has poor treatment options.

Original languageEnglish
Article number825
Number of pages8
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 16 Jan 2018
Externally publishedYes

Cite this

Busuttil, R. A., Liu, D. S., Di Costanzo, N., Schröder, J., Mitchell, C., & Boussioutas, A. (2018). An orthotopic mouse model of gastric cancer invasion and metastasis. Scientific Reports, 8(1), [825]. https://doi.org/10.1038/s41598-017-19025-y
Busuttil, Rita A. ; Liu, David S. ; Di Costanzo, Natasha ; Schröder, Jan ; Mitchell, Catherine ; Boussioutas, Alex. / An orthotopic mouse model of gastric cancer invasion and metastasis. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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Busuttil, RA, Liu, DS, Di Costanzo, N, Schröder, J, Mitchell, C & Boussioutas, A 2018, 'An orthotopic mouse model of gastric cancer invasion and metastasis', Scientific Reports, vol. 8, no. 1, 825. https://doi.org/10.1038/s41598-017-19025-y

An orthotopic mouse model of gastric cancer invasion and metastasis. / Busuttil, Rita A.; Liu, David S.; Di Costanzo, Natasha; Schröder, Jan; Mitchell, Catherine; Boussioutas, Alex.

In: Scientific Reports, Vol. 8, No. 1, 825, 16.01.2018.

Research output: Contribution to journalArticleResearchpeer-review

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