An optical tweezers - Based immunosensor for detection of femtomoles-per-liter concentrations of antigens

Kristian Helmerson, Rani Kishore, William D. Phillips, Howard H. Weetall

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8 Citations (Scopus)

Abstract

We used optical tweezers - optical trapping with focused laser beams - to pull microspheres coated with antigens off of an antibody-coated surface. Using this technique, we could quantify the force required to separate antigen to antibody bonds. At very low surface density of antigen, we were able to detect the single antigen to antibody binding. The force required to break the antigen-antibody bonds and pull the microsphere off the surface was shown to increase monotonically with increasing surface density of antigens. Using the force determination as a transducer, we were able to detect concentrations of free antigens in solution as small as 10-15 mol/L in a competitive binding assay.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume96
Issue number1-3
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Keywords

  • Antibody
  • Antigen
  • Assay
  • BSA
  • Immunosensor
  • Laser trap
  • Optical tweezers

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