An investigation on platelet transport during thrombus formation at micro-scale stenosis

Francisco J Tovar-Lopez, Gary Rosengarten, Mahyar Nasabi, Vijay Sivan, Khashayar Khoshmanesh, Shaun Jackson, Arnan Mitchell, Warwick Scott Nesbitt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This paper reports on an investigation of mass transport of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. Using a microfluidic flow focusing platform we investigate the blood flow streams that principally contribute to platelet aggregation under shear micro-gradient conditions. We demonstrate that relatively thin surface streams located at the channel wall are the primary contributor of platelets to the developing aggregate under shear gradient conditions. Furthermore we delineate a role for red blood cell hydrodynamic lift forces in driving enhanced advection of platelets to the stenosis wall and surface of developing aggregates. We show that this novel microfluidic platform can be effectively used to study the role of mass transport phenomena driving platelet recruitment and aggregate formation and believe that this approach will lead to a greater understanding of the mechanisms underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke
Original languageEnglish
Pages (from-to)1 - 11
Number of pages11
JournalPLoS ONE
Volume8
Issue number10 (Art. ID: e74123)
DOIs
Publication statusPublished - 2013

Cite this

Tovar-Lopez, Francisco J ; Rosengarten, Gary ; Nasabi, Mahyar ; Sivan, Vijay ; Khoshmanesh, Khashayar ; Jackson, Shaun ; Mitchell, Arnan ; Nesbitt, Warwick Scott. / An investigation on platelet transport during thrombus formation at micro-scale stenosis. In: PLoS ONE. 2013 ; Vol. 8, No. 10 (Art. ID: e74123). pp. 1 - 11.
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Tovar-Lopez, FJ, Rosengarten, G, Nasabi, M, Sivan, V, Khoshmanesh, K, Jackson, S, Mitchell, A & Nesbitt, WS 2013, 'An investigation on platelet transport during thrombus formation at micro-scale stenosis' PLoS ONE, vol. 8, no. 10 (Art. ID: e74123), pp. 1 - 11. https://doi.org/10.1371/journal.pone.0074123

An investigation on platelet transport during thrombus formation at micro-scale stenosis. / Tovar-Lopez, Francisco J; Rosengarten, Gary; Nasabi, Mahyar; Sivan, Vijay; Khoshmanesh, Khashayar; Jackson, Shaun; Mitchell, Arnan; Nesbitt, Warwick Scott.

In: PLoS ONE, Vol. 8, No. 10 (Art. ID: e74123), 2013, p. 1 - 11.

Research output: Contribution to journalArticleResearchpeer-review

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Tovar-Lopez FJ, Rosengarten G, Nasabi M, Sivan V, Khoshmanesh K, Jackson S et al. An investigation on platelet transport during thrombus formation at micro-scale stenosis. PLoS ONE. 2013;8(10 (Art. ID: e74123)):1 - 11. https://doi.org/10.1371/journal.pone.0074123