An investigation of clinical and immunological events following repeated aerodigestive tract challenge infections with live Mycobacterium bovis Bacille Calmette Guérin

Fernanda Schreiber, Zhiming Huo, Rafaela Giemza, Maria Woodrow, Nicola Fenner, Zoe Stephens, Gordon Dougan, Steven Prideaux, Luiz R R Castello-Branco, David J M Lewis

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Bacille Calmette Guérin substrain Moreau Rio de Janeiro is an attenuated strain of Mycobacterium bovis that has been used extensively as an oral tuberculosis vaccine. We assessed its potential as a challenge model to study clinical and immunological events following repeated mycobacterial gut infection. Seven individuals received three oral challenges with approximately 107 viable bacilli. Clinical symptoms, T-cell responses and gene expression patterns in peripheral blood were monitored. Clinical symptoms were relatively mild and declined following each oral challenge. Delayed T-cell responses were observed, and limited differential gene expression detected by microarrays. Oral challenge with BCG Moreau Rio de Janeiro vaccine was immunogenic in healthy volunteers, limiting its potential to explore clinical innate immune responses, but with low reactogenicity.

Original languageEnglish
Pages (from-to)5427-5431
Number of pages5
JournalVaccine
Volume28
Issue number33
DOIs
Publication statusPublished - 1 Jul 2010
Externally publishedYes

Keywords

  • BCG
  • Innate immunity
  • Oral challenge

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