An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal-type V2-receptor. An active role for receptor immobilization in down-regulation?

David A. Jans, Reiner Peters, Falk Fahrenholz

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5 Citations (Scopus)

Abstract

Lateral mobility of the vasopressin renal-type V2-receptor was investigated in LLC-PK1, porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine-labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobility and ligand internalization. The V2-receptor mobile fraction diminished from 0.9 to 0.43 over 60 min at 37°C. whereas the apparent lateral diffusion coefficient remained essentially unchanged (2-3 × 10-10 cm2 s). Interestingly, the fraction of immobile V2-receptors corresponded exactly with the fraction of internalized receptors, implying a functional relationship. These observations together with comparable results reported for other polypeptide hormone receptors indicate a possible machanistic role for receptor immobilization in the desensitization of hormonal response.

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalFEBS Letters
Volume274
Issue number1-2
DOIs
Publication statusPublished - 12 Nov 1990
Externally publishedYes

Keywords

  • Internalization
  • Lateral mobility
  • Vasopressin renal V-type receptor: Photobleaching

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