An interface coassembly in biliquid phase: toward core-shell magnetic mesoporous silica microspheres with tunable pore size

Qin Yue, Jialuo Li, Wei Luo, Yu Zhang, Ahmed A. Elzatahry, Xiqing Wang, Chun Wang, Wei Li, Xiaowei Cheng, Abdulaziz Alghamdi, Aboubakr M. Abdullah, Yonghui Deng, Dongyuan Zhao

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228 Citations (Scopus)


Core–shell magnetic mesoporous silica microspheres (Magn-MSMs) with tunable large mesopores in the shell are highly desired in biocatalysis, magnetic bioseparation, and enrichment. In this study, a shearing assisted interface coassembly in n-hexane/water biliquid systems is developed to synthesize uniform Magn-MSMs with magnetic core and mesoporous silica shell for an efficient size-selective biocatalysis. The synthesis features the rational control over the electrostatic interaction among cationic surfactant molecules, silicate oligomers, and Fe3O4@RF microspheres (RF: resorcinol formaldehyde) in the presence of shearing-regulated solubilization of n-hexane in surfactant micelles. Through this multicomponent interface coassembly, surfactant-silica mesostructured composite has been uniformly deposited on the Fe3O4@RF microspheres, and core–shell Magn-MSMs are obtained after removing the surfactant and n-hexane. The obtained Magn-MSMs possess excellent water dispersibility, uniform diameter (600 nm), large and tunable perpendicular mesopores (5.0–9.0 nm), high surface area (498–623 m2/g), large pore volume (0.91–0.98 cm3/g), and high magnetization (34.5–37.1 emu/g). By utilization of their large and open mesopores, Magn-MSMs with a pore size of about 9.0 nm have been demonstrated to be able to immobilize a large bioenzyme (trypsin with size of 4.0 nm) with a high loading capacity of ∼97 μg/mg via chemically binding. Magn-MSMs with immobilized trypsin exhibit an excellent convenient and size selective enzymolysis of low molecular proteins in the mixture of proteins of different sizes and a good recycling performance by using the magnetic separability of the microspheres.
Original languageEnglish
Pages (from-to)13282-13289
Number of pages8
JournalJournal of the American Chemical Society
Issue number41
Publication statusPublished - 21 Oct 2015
Externally publishedYes

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