An innovative triple immunogold labeling method to investigate the hemopoietic stem cell niche in situ.

Sarah Lynne Ellis, Brenda Williams, Stephen Asquith, Ivan Bertoncello, Susan K. Nilsson

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The ultrastructural study of rare cells within their niche in situ is very difficult. We have developed a method for locating individual transplanted cells and simultaneously identifying and analyzing the molecules and cellular phenotypes surrounding them in situ using transmission electron microscopy. This innovative method involves triple immunogold labeling combined with serial ultrathin sectioning. We demonstrate the validity of this approach by examining the niche of individual transplanted cells from a population highly enriched for hemopoietic stem cells and the ultrastructural expression of two key stem cell regulatory molecules, hyaluronic acid and osteopontin. In addition, we describe the phenotypes of the surrounding cells.

Original languageEnglish
Pages (from-to)403-414
Number of pages12
JournalMicroscopy and Microanalysis
Volume15
Issue number5
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Cite this