An influenza virus-inspired polymer system for the timed release of siRNA

Nghia Truong Phuoc, Wenyi Gu, Indira Prasadam, Zhongfan Jia, Ross William Crawford, Yin Xiao, Michael J Monteiro

Research output: Contribution to journalArticleResearchpeer-review

120 Citations (Scopus)

Abstract

Small interfering RNA silences specific genes by interfering with mRNA translation, and acts to modulate or inhibit specific biological pathways; a therapy that holds great promise in the cure of many diseases. However, the naked small interfering RNA is susceptible to degradation by plasma and tissue nucleases and due to its negative charge unable to cross the cell membrane. Here we report a new polymer carrier designed to mimic the influenza virus escape mechanism from the endosome, followed by a timed release of the small interfering RNA in the cytosol through a self-catalyzed polymer degradation process. Our polymer changes to a negatively charged and non-toxic polymer after the release of small interfering RNA, presenting potential for multiple repeat doses and long-term treatment of diseases.
Original languageEnglish
Pages (from-to)1 - 7
Number of pages7
JournalNature Communications
Volume4
Issue number1902
DOIs
Publication statusPublished - 2013
Externally publishedYes

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