TY - JOUR
T1 - An in vitro study of the antifungal efficacy of zinc oxide nanoparticles against Saccharomyces cerevisiae
AU - Tan, Eng Pei
AU - Djearamane, Sinouvassane
AU - Wong, Ling Shing
AU - Rajamani, Ranjithkumar
AU - Tanislaus Antony, Anto Cordelia
AU - Subbaih, Suresh Kumar
AU - Janakiraman, Ashok Kumar
AU - Aminuzzaman, Mohammod
AU - Subramaniyan, Vetriselvan
AU - Sekar, Mahendran
AU - Selvaraj, Siddharthan
N1 - Funding Information:
This research work was funded by Universiti Tunku Abdul Rahman Research Fund (IPSR/RMC/UTARRF/2020-C2/S06) and Ministry of Education, Malaysia (Grant No. FRGS-1-2014-SG03-INTI-02-1).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - Zinc oxide nanoparticles (ZnO NPs) are widely used in biomedical applications due to their antimicrobial and antioxidant properties. The objective of the present study was to determine the antifungal activity of ZnO NPs against the yeast Saccharomyces cerevisiae. The turbidity test results showed a significant (p < 0.05) dose-dependent growth inhibitory effect of ZnO NPs on S. cerevisiae as the growth inhibition increased from 7.04 ± 0.64 to 70.30 ± 3.19% as the concentration of ZnO NPs increased from 5 to 150 μg/mL. The scanning microscopy images evidenced the morphological alterations such as regional invagination, pitting, cracks, wrinkles, and cell wall rupture in the yeast cells treated with ZnO NPs. In addition, the FTIR spectrum revealed the possible involvement of hydroxyl, alkene, amides, carbonyl, and phosphate groups from polysaccharides, polypeptides, phospholipids, and ergosterol of the yeast cells wall for binding of ZnO NPs on the cell surface. The present study has demonstrated the antifungal activity of ZnO NPs on S. cerevisiae through growth inhibition and the morphological damages resulting from the treatment of ZnO NPs.
AB - Zinc oxide nanoparticles (ZnO NPs) are widely used in biomedical applications due to their antimicrobial and antioxidant properties. The objective of the present study was to determine the antifungal activity of ZnO NPs against the yeast Saccharomyces cerevisiae. The turbidity test results showed a significant (p < 0.05) dose-dependent growth inhibitory effect of ZnO NPs on S. cerevisiae as the growth inhibition increased from 7.04 ± 0.64 to 70.30 ± 3.19% as the concentration of ZnO NPs increased from 5 to 150 μg/mL. The scanning microscopy images evidenced the morphological alterations such as regional invagination, pitting, cracks, wrinkles, and cell wall rupture in the yeast cells treated with ZnO NPs. In addition, the FTIR spectrum revealed the possible involvement of hydroxyl, alkene, amides, carbonyl, and phosphate groups from polysaccharides, polypeptides, phospholipids, and ergosterol of the yeast cells wall for binding of ZnO NPs on the cell surface. The present study has demonstrated the antifungal activity of ZnO NPs on S. cerevisiae through growth inhibition and the morphological damages resulting from the treatment of ZnO NPs.
KW - FTIR
KW - growth inhibition
KW - morphological alterations
KW - scanning electron microscopy
KW - yeast cells
UR - http://www.scopus.com/inward/record.url?scp=85144859604&partnerID=8YFLogxK
U2 - 10.3390/coatings12121988
DO - 10.3390/coatings12121988
M3 - Article
AN - SCOPUS:85144859604
SN - 2079-6412
VL - 12
JO - Coatings
JF - Coatings
IS - 12
M1 - 1988
ER -