An in vitro model of allergen-dependent IgE synthesis by human B lymphocytes: Comparison of the response of an atopic and a non-atopic individual to Dermatophagoides spp. (house dust mite)

R. E. O'Hehir, V. Bal, D. Quint, R. Moqbel, A. B. Kay, E. D. Zanders, J. R. Lamb

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Abstract

An allergen-dependent in vitro model of immunoglobulin E (IgE) synthesis by human B cells is reported. Using this model, it is demonstrated that polyclonal T cells and CD4+ Dermatophagoides spp. (house dust mite)-specific T-cell clones derived from an atopic, house dust mite (HDM)-allergic individual are able to support IgE synthesis by autologous B cells. The helper activity was interleukin-4 (IL-4) dependent as only cloned T cells expressing detectable mRNA for IL-4 were able to induce IgE synthesis without the addition of exogenous IL-4. Peripheral and cloned T cells reactive with HDM could also be identified from a non-atopic individual but neither population was able to support IgE production even in the presence of exogenous IL-4.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalImmunology
Volume66
Issue number4
Publication statusPublished - 1 Jan 1989
Externally publishedYes

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