An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mouse Cell Co-cultures

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Abstract

Acute pancreatitis is a serious inflammatory disease of the pancreas that can lead to lung injury. Despite extensive research, the mechanisms underlying this complication are ill-defined. In recent years, in vitro co-culture systems have emerged as powerful tools for studying complex interactions between different cell types in disease. In the context of pancreatitis, pancreatic acinar epithelial cells produce and secrete digestive enzymes, and their cellular damage, death, and/or dysfunction is a major contributing factor to the onset of pancreatitis. Here, in this chapter we describe a co-culture system of acinar cells and lung epithelial progenitor/stem cells to model for lung injury associated with pancreatitis.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter6
Pages71-80
Number of pages10
Edition2nd
ISBN (Electronic)9781071633311
ISBN (Print)9781071633304
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2691
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Acinar cells
  • Acute lung injury
  • In vitro co-cultures
  • Lung stem cell niche
  • Organoids
  • Pancreatitis

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