An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture

Rosemary L. Sparrow; Neil Williams

doi:10.1016/0022-1759(86)90523-5

An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture

Rosemary L. Sparrow
, Neil Williams

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.

Original language	English
Pages (from-to)	145-148
Number of pages	4
Journal	Journal of Immunological Methods
Volume	87
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90523-5
Publication status	Published - 13 Mar 1986
Externally published	Yes

Keywords

Agar cell culture
Immunoperoxidase
Monoclonal antibody

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10.1016/0022-1759(86)90523-5

Cite this

@article{1e4ff3b8e58e4812b0b26de1f7d91b82,

title = "An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture",

abstract = "An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.",

keywords = "Agar cell culture, Immunoperoxidase, Monoclonal antibody",

author = "Sparrow, \{Rosemary L.\} and Neil Williams",

year = "1986",

month = mar,

day = "13",

doi = "10.1016/0022-1759(86)90523-5",

language = "English",

volume = "87",

pages = "145--148",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture

AU - Sparrow, Rosemary L.

AU - Williams, Neil

PY - 1986/3/13

Y1 - 1986/3/13

N2 - An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.

AB - An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.

KW - Agar cell culture

KW - Immunoperoxidase

KW - Monoclonal antibody

UR - https://www.scopus.com/pages/publications/0022608388

U2 - 10.1016/0022-1759(86)90523-5

DO - 10.1016/0022-1759(86)90523-5

M3 - Article

C2 - 3512725

AN - SCOPUS:0022608388

SN - 0022-1759

VL - 87

SP - 145

EP - 148

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture

Abstract

Keywords

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