An in situ immunoperoxidase staining procedure for human cell colonies grown in semi-solid agar culture

Rosemary L. Sparrow, Neil Williams

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An adaptation of the immunoperoxidase staining procedure is described which enables the detection of antigens expressed by human hemopoietic cells grown in semi-solid agar culture. Positive staining was achieved using several different monoclonal antibodies recognizing an array of cell surface antigens on developing myeloid cells.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalJournal of Immunological Methods
Issue number2
Publication statusPublished - 13 Mar 1986
Externally publishedYes


  • Agar cell culture
  • Immunoperoxidase
  • Monoclonal antibody

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