Abstract
| Language | English |
|---|---|
| Pages | 49-53 |
| Number of pages | 5 |
| Journal | Stem Cell Research |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jul 2016 |
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An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3. / Alaei, S.; Knaupp, A.S; Lim, S.M.; Chen, J.; Holmes, M.L.; Ankö, M-L; Nefzger, C.M.; Polo, J. M.
In: Stem Cell Research, Vol. 17, No. 1, 01.07.2016, p. 49-53.Research output: Contribution to journal › Article
TY - JOUR
T1 - An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3
AU - Alaei,S.
AU - Knaupp,A.S
AU - Lim,S.M.
AU - Chen,J.
AU - Holmes,M.L.
AU - Ankö,M-L
AU - Nefzger,C.M.
AU - Polo,J. M.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Reprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in a low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>. 9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process.
AB - Reprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in a low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>. 9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process.
UR - http://www.scopus.com/inward/record.url?scp=84969753035&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2016.05.008
DO - 10.1016/j.scr.2016.05.008
M3 - Article
VL - 17
SP - 49
EP - 53
JO - Stem Cell Research
T2 - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
IS - 1
ER -