An Fgf-Shh positive feedback loop drives growth in developing unpaired fins

M. Brent Hawkins; David Jandzik; Frank J. Tulenko; Amanda N. Cass; Tetsuya Nakamura; Neil H. Shubin; Marcus C. Davis; David W. Stock

doi:10.1073/pnas.2120150119

An Fgf-Shh positive feedback loop drives growth in developing unpaired fins

M. Brent Hawkins, David Jandzik, Frank J. Tulenko, Amanda N. Cass, Tetsuya Nakamura, Neil H. Shubin, Marcus C. Davis, David W. Stock

Australian Regenerative Medicine Institute

Research output: Contribution to journal › Article › Other › peer-review

3 Citations (Scopus)

Abstract

The origin and diversification of appendage types is a central question in vertebrate evolution. Understanding the genetic mechanisms that underlie fin and limb development can reveal relationships between different appendages. Here we demonstrate, using chemical genetics, a mutually agonistic interaction between Fgf and Shh genes in the developing dorsal fin of the channel catfish, Ictalurus punctatus. We also find that Fgf8 and Shh orthologs are expressed in the apical ectodermal ridge and zone of polarizing activity, respectively, in the median fins of representatives from other major vertebrate lineages. These findings demonstrate the importance of this feedback loop in median fins and offer developmental evidence for a median fin-first scenario for vertebrate paired appendage origins.

Original language	English
Article number	e2120150119
Number of pages	3
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	119
Issue number	10
DOIs	https://doi.org/10.1073/pnas.2120150119
Publication status	Published - 8 Mar 2022

Keywords

Evolutionary developmental biology
Fibroblast growth factor signaling
Hedgehog signaling
Median fin
Unpaired fin

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10.1073/pnas.2120150119Licence: CC BY-NC-ND

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title = "An Fgf-Shh positive feedback loop drives growth in developing unpaired fins",

abstract = "The origin and diversification of appendage types is a central question in vertebrate evolution. Understanding the genetic mechanisms that underlie fin and limb development can reveal relationships between different appendages. Here we demonstrate, using chemical genetics, a mutually agonistic interaction between Fgf and Shh genes in the developing dorsal fin of the channel catfish, Ictalurus punctatus. We also find that Fgf8 and Shh orthologs are expressed in the apical ectodermal ridge and zone of polarizing activity, respectively, in the median fins of representatives from other major vertebrate lineages. These findings demonstrate the importance of this feedback loop in median fins and offer developmental evidence for a median fin-first scenario for vertebrate paired appendage origins.",

keywords = "Evolutionary developmental biology, Fibroblast growth factor signaling, Hedgehog signaling, Median fin, Unpaired fin",

author = "Hawkins, {M. Brent} and David Jandzik and Tulenko, {Frank J.} and Cass, {Amanda N.} and Tetsuya Nakamura and Shubin, {Neil H.} and Davis, {Marcus C.} and Stock, {David W.}",

note = "Funding Information: ACKNOWLEDGMENTS. The catfish illustration was based on a photograph kindly provided by Melissa McGaw. Kristen McDaniel and Rebecca Gonzalez helped process catfish eggs. Joseph M. Sanchez Jr. helped process catfish eggs and provided fish care. Alexander Cruz provided space and equipment for cat-fish husbandry. This work was supported by the NSF grants IBN-0446720, IOS-1121855, and IOS-1755305 (to D.W.S.); NSF grant IOS-1853949 (to M.C.D.); the Scientific Grant Agency of Slovak Republic (Vedeck{\'a} Grantov{\'a} Agent{\'u}ra MS≤VVaS≤ SR a SAV [VEGA]) grant 1/0450/21 (to D.J.); and the Brinson Foundation (to N.H.S.). Publisher Copyright: {\textcopyright} 2022 National Academy of Sciences. All rights reserved.",

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AU - Hawkins, M. Brent

AU - Jandzik, David

AU - Tulenko, Frank J.

AU - Cass, Amanda N.

AU - Nakamura, Tetsuya

AU - Shubin, Neil H.

AU - Davis, Marcus C.

AU - Stock, David W.

N1 - Funding Information: ACKNOWLEDGMENTS. The catfish illustration was based on a photograph kindly provided by Melissa McGaw. Kristen McDaniel and Rebecca Gonzalez helped process catfish eggs. Joseph M. Sanchez Jr. helped process catfish eggs and provided fish care. Alexander Cruz provided space and equipment for cat-fish husbandry. This work was supported by the NSF grants IBN-0446720, IOS-1121855, and IOS-1755305 (to D.W.S.); NSF grant IOS-1853949 (to M.C.D.); the Scientific Grant Agency of Slovak Republic (Vedecká Grantová Agentúra MS≤VVaS≤ SR a SAV [VEGA]) grant 1/0450/21 (to D.J.); and the Brinson Foundation (to N.H.S.). Publisher Copyright: © 2022 National Academy of Sciences. All rights reserved.

PY - 2022/3/8

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N2 - The origin and diversification of appendage types is a central question in vertebrate evolution. Understanding the genetic mechanisms that underlie fin and limb development can reveal relationships between different appendages. Here we demonstrate, using chemical genetics, a mutually agonistic interaction between Fgf and Shh genes in the developing dorsal fin of the channel catfish, Ictalurus punctatus. We also find that Fgf8 and Shh orthologs are expressed in the apical ectodermal ridge and zone of polarizing activity, respectively, in the median fins of representatives from other major vertebrate lineages. These findings demonstrate the importance of this feedback loop in median fins and offer developmental evidence for a median fin-first scenario for vertebrate paired appendage origins.

AB - The origin and diversification of appendage types is a central question in vertebrate evolution. Understanding the genetic mechanisms that underlie fin and limb development can reveal relationships between different appendages. Here we demonstrate, using chemical genetics, a mutually agonistic interaction between Fgf and Shh genes in the developing dorsal fin of the channel catfish, Ictalurus punctatus. We also find that Fgf8 and Shh orthologs are expressed in the apical ectodermal ridge and zone of polarizing activity, respectively, in the median fins of representatives from other major vertebrate lineages. These findings demonstrate the importance of this feedback loop in median fins and offer developmental evidence for a median fin-first scenario for vertebrate paired appendage origins.

KW - Evolutionary developmental biology

KW - Fibroblast growth factor signaling

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KW - Unpaired fin

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An Fgf-Shh positive feedback loop drives growth in developing unpaired fins

Abstract

Keywords

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