An expressional system of human cytochrome P-450 CYP1A1 gene transcription

Hui Wang, Gary Williamson, Ren Xiu Peng

Research output: Contribution to journalArticleResearchpeer-review

Abstract

AIM: To explore an expressional system of human cytochrome P-450 CYP1A1 (CYP1A1) gene transcription. METHODS: The plasmid pMC 6.3 K containing human CYP1A1 promoter was transiently transfected into Hep G2 cells. The expression of chloramphenical acetyltransferase (CAT) reporter gene was detected by ELISA. RESULTS: Both the CAT expression and CYP1A1 activity increased with the concentrations of β-naphthoflavone from 2.5 to 10 μmol·L-1. At 10 μmol · L-1 of β-naphthoflavone, the levels of CAT and CYP1A1 were 94- fold and 2.8-fold those of the corresponding control, respectively. Using this method, the study of 8 glucosinolates with various side chains on the induction of CYP1A1 gene transcription showed that none of the parent glucosinolates increased CAT expression, whereas the breakdown products of indol-3-yl-methyl glucosinolate (glucobrassicin), rather than indole-3- carbinol, increased the CAT expression. CONCLUSION: The CYP1A1 gene transcription system was more reliable and sensitive.

Original languageEnglish
Pages (from-to)429-432
Number of pages4
JournalActa Pharmacologica Sinica
Volume19
Issue number5
Publication statusPublished - 1 Sep 1998
Externally publishedYes

Keywords

  • Brassica
  • Chloramphenicol acetyltransferase
  • Cytochrome P-450 CYP1A1
  • Flavones
  • Glucosinolates
  • Hepatoblastoma
  • Plasmids
  • Transfection

Cite this