An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells

Lev M Kats, Kate Marie Fernandez, Fiona K Glenister, Susann Herrmann, Donna W Buckingham, Ghizal Siddiqui, Laveena Sharma, Rebecca S Bamert, Isabelle S Lucet, Micheline Guillotte, Odile Mercereau-Puijalon, Brian M Cooke

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25 Citations (Scopus)

Abstract

Alteration of the adhesive and mechanical properties of red blood cells caused by infection with the malaria parasite Plasmodium falciparum underpin both its survival and extreme pathogenicity. A unique family of parasite putative exported kinases, collectively called FIKK (Phenylalanine (F) - Isoleucine (I) - Lysine (K) - Lysine (K)), has recently been implicated in these pathophysiological processes, however, their precise function in P. falciparum-infected red blood cells or their likely role in malaria pathogenesis remain unknown. Here, for the first time, we demonstrate that one member of the FIKK family, FIKK4.2, can function as an active kinase and is localised in a novel and distinct compartment of the parasite-infected red blood cell which we have called K-dots. Notably, targeted disruption of the gene encoding FIKK4.2 (fikk4.2) dramatically alters the parasite s ability to modify and remodel the red blood cells in which it multiplies. Specifically, red blood cells infected with fikk4.2 knockout parasites were significantly less rigid and less adhesive when compared with red blood cells infected with normal parasites from which the transgenic clones had been derived, despite expressing similar levels of the major cytoadhesion ligand, PfEMP1, on the red blood cell surface. Notably, these changes were accompanied by dramatically altered knob-structures on infected red blood cells that play a key role in cytoadhesion which is responsible for much of the pathogenesis associated with falciparum malaria. Taken together, our data identifies FIKK4.2 as an important kinase in the pathogenesis of P. falciparum malaria and strengthens the attractiveness of FIKK kinases as targets for the development of novel next-generation anti-malaria drugs.
Original languageEnglish
Pages (from-to)319 - 328
Number of pages10
JournalInternational Journal for Parasitology
Volume44
Issue number5
DOIs
Publication statusPublished - 2014

Cite this

Kats, Lev M ; Fernandez, Kate Marie ; Glenister, Fiona K ; Herrmann, Susann ; Buckingham, Donna W ; Siddiqui, Ghizal ; Sharma, Laveena ; Bamert, Rebecca S ; Lucet, Isabelle S ; Guillotte, Micheline ; Mercereau-Puijalon, Odile ; Cooke, Brian M. / An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells. In: International Journal for Parasitology. 2014 ; Vol. 44, No. 5. pp. 319 - 328.
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title = "An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells",
abstract = "Alteration of the adhesive and mechanical properties of red blood cells caused by infection with the malaria parasite Plasmodium falciparum underpin both its survival and extreme pathogenicity. A unique family of parasite putative exported kinases, collectively called FIKK (Phenylalanine (F) - Isoleucine (I) - Lysine (K) - Lysine (K)), has recently been implicated in these pathophysiological processes, however, their precise function in P. falciparum-infected red blood cells or their likely role in malaria pathogenesis remain unknown. Here, for the first time, we demonstrate that one member of the FIKK family, FIKK4.2, can function as an active kinase and is localised in a novel and distinct compartment of the parasite-infected red blood cell which we have called K-dots. Notably, targeted disruption of the gene encoding FIKK4.2 (fikk4.2) dramatically alters the parasite s ability to modify and remodel the red blood cells in which it multiplies. Specifically, red blood cells infected with fikk4.2 knockout parasites were significantly less rigid and less adhesive when compared with red blood cells infected with normal parasites from which the transgenic clones had been derived, despite expressing similar levels of the major cytoadhesion ligand, PfEMP1, on the red blood cell surface. Notably, these changes were accompanied by dramatically altered knob-structures on infected red blood cells that play a key role in cytoadhesion which is responsible for much of the pathogenesis associated with falciparum malaria. Taken together, our data identifies FIKK4.2 as an important kinase in the pathogenesis of P. falciparum malaria and strengthens the attractiveness of FIKK kinases as targets for the development of novel next-generation anti-malaria drugs.",
author = "Kats, {Lev M} and Fernandez, {Kate Marie} and Glenister, {Fiona K} and Susann Herrmann and Buckingham, {Donna W} and Ghizal Siddiqui and Laveena Sharma and Bamert, {Rebecca S} and Lucet, {Isabelle S} and Micheline Guillotte and Odile Mercereau-Puijalon and Cooke, {Brian M}",
year = "2014",
doi = "10.1016/j.ijpara.2014.01.003",
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Kats, LM, Fernandez, KM, Glenister, FK, Herrmann, S, Buckingham, DW, Siddiqui, G, Sharma, L, Bamert, RS, Lucet, IS, Guillotte, M, Mercereau-Puijalon, O & Cooke, BM 2014, 'An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells', International Journal for Parasitology, vol. 44, no. 5, pp. 319 - 328. https://doi.org/10.1016/j.ijpara.2014.01.003

An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells. / Kats, Lev M; Fernandez, Kate Marie; Glenister, Fiona K; Herrmann, Susann; Buckingham, Donna W; Siddiqui, Ghizal; Sharma, Laveena; Bamert, Rebecca S; Lucet, Isabelle S; Guillotte, Micheline; Mercereau-Puijalon, Odile; Cooke, Brian M.

In: International Journal for Parasitology, Vol. 44, No. 5, 2014, p. 319 - 328.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells

AU - Kats, Lev M

AU - Fernandez, Kate Marie

AU - Glenister, Fiona K

AU - Herrmann, Susann

AU - Buckingham, Donna W

AU - Siddiqui, Ghizal

AU - Sharma, Laveena

AU - Bamert, Rebecca S

AU - Lucet, Isabelle S

AU - Guillotte, Micheline

AU - Mercereau-Puijalon, Odile

AU - Cooke, Brian M

PY - 2014

Y1 - 2014

N2 - Alteration of the adhesive and mechanical properties of red blood cells caused by infection with the malaria parasite Plasmodium falciparum underpin both its survival and extreme pathogenicity. A unique family of parasite putative exported kinases, collectively called FIKK (Phenylalanine (F) - Isoleucine (I) - Lysine (K) - Lysine (K)), has recently been implicated in these pathophysiological processes, however, their precise function in P. falciparum-infected red blood cells or their likely role in malaria pathogenesis remain unknown. Here, for the first time, we demonstrate that one member of the FIKK family, FIKK4.2, can function as an active kinase and is localised in a novel and distinct compartment of the parasite-infected red blood cell which we have called K-dots. Notably, targeted disruption of the gene encoding FIKK4.2 (fikk4.2) dramatically alters the parasite s ability to modify and remodel the red blood cells in which it multiplies. Specifically, red blood cells infected with fikk4.2 knockout parasites were significantly less rigid and less adhesive when compared with red blood cells infected with normal parasites from which the transgenic clones had been derived, despite expressing similar levels of the major cytoadhesion ligand, PfEMP1, on the red blood cell surface. Notably, these changes were accompanied by dramatically altered knob-structures on infected red blood cells that play a key role in cytoadhesion which is responsible for much of the pathogenesis associated with falciparum malaria. Taken together, our data identifies FIKK4.2 as an important kinase in the pathogenesis of P. falciparum malaria and strengthens the attractiveness of FIKK kinases as targets for the development of novel next-generation anti-malaria drugs.

AB - Alteration of the adhesive and mechanical properties of red blood cells caused by infection with the malaria parasite Plasmodium falciparum underpin both its survival and extreme pathogenicity. A unique family of parasite putative exported kinases, collectively called FIKK (Phenylalanine (F) - Isoleucine (I) - Lysine (K) - Lysine (K)), has recently been implicated in these pathophysiological processes, however, their precise function in P. falciparum-infected red blood cells or their likely role in malaria pathogenesis remain unknown. Here, for the first time, we demonstrate that one member of the FIKK family, FIKK4.2, can function as an active kinase and is localised in a novel and distinct compartment of the parasite-infected red blood cell which we have called K-dots. Notably, targeted disruption of the gene encoding FIKK4.2 (fikk4.2) dramatically alters the parasite s ability to modify and remodel the red blood cells in which it multiplies. Specifically, red blood cells infected with fikk4.2 knockout parasites were significantly less rigid and less adhesive when compared with red blood cells infected with normal parasites from which the transgenic clones had been derived, despite expressing similar levels of the major cytoadhesion ligand, PfEMP1, on the red blood cell surface. Notably, these changes were accompanied by dramatically altered knob-structures on infected red blood cells that play a key role in cytoadhesion which is responsible for much of the pathogenesis associated with falciparum malaria. Taken together, our data identifies FIKK4.2 as an important kinase in the pathogenesis of P. falciparum malaria and strengthens the attractiveness of FIKK kinases as targets for the development of novel next-generation anti-malaria drugs.

UR - http://www.sciencedirect.com/science/article/pii/S0020751914000289

U2 - 10.1016/j.ijpara.2014.01.003

DO - 10.1016/j.ijpara.2014.01.003

M3 - Article

VL - 44

SP - 319

EP - 328

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 5

ER -