TY - JOUR
T1 - An examination of cardiovascular collapse induced by eastern brown snake (Pseudonaja textilis) venom
AU - Chaisakul, Janeyuth
AU - Isbister, Geoffrey K
AU - Kuruppu, Don Monath Sanjaya
AU - Konstantakopoulos, Nicki
AU - Hodgson, Wayne Clarence
PY - 2013
Y1 - 2013
N2 - The Pseudonaja genus (Brown snakes) is widely distributed across Australia and bites account for significant mortality. Venom-induced consumption coagulopathy (VICC) and, less often, early cardiovascular collapse occur following envenoming by these snakes. We have previously examined possible mechanism(s) behind the early cardiovascular collapse following Papuan taipan (Oxyuranus scutellatus) envenoming. In the present study, we investigate early cardiovascular collapse in anaesthetized rats following administration of eastern brown snake (Pseudonaja textilis) venom, and prevention of this effect with prior administration of priming doses (i.e. doses of venom which caused a transient hypotensive response) of venom. P. textilis venom (5-10mug/kg, i.v.) induced cardiovascular collapse in anaesthetized rats, characterized by a rapid decrease in systolic blood pressure until non recordable. Prior administration of priming doses of P. textilis venom (2 and 3mug/kg) or, at least, 4-5 doses of O. scutellatus (2mug/kg, i.v.) or Daboia russelii limitis (20mug/kg, i.v.) venoms prevented cardiovascular collapse induced by P. textilis venom. Moreover, early collapse was also inhibited by prior administration of 2 discrete doses of Acanthophis rugosus venom. Prior administration of commercial polyvalent snake antivenom (500-3000 units/kg, i.v.) or heparin (300 units/kg, i.v.) also inhibited P. textilis venom-induced cardiovascular collapse. Our results indicate that P. textilis venom-induced cardiovascular collapse can be prevented by prior administration of sub lethal doses of venom from P. textilis, O. scutellatus, A. rugosus and D. russelii limitis. This suggests that sudden cardiovascular collapse following envenoming is likely to involve a common mechanism/pathway activated by different snake venoms.
AB - The Pseudonaja genus (Brown snakes) is widely distributed across Australia and bites account for significant mortality. Venom-induced consumption coagulopathy (VICC) and, less often, early cardiovascular collapse occur following envenoming by these snakes. We have previously examined possible mechanism(s) behind the early cardiovascular collapse following Papuan taipan (Oxyuranus scutellatus) envenoming. In the present study, we investigate early cardiovascular collapse in anaesthetized rats following administration of eastern brown snake (Pseudonaja textilis) venom, and prevention of this effect with prior administration of priming doses (i.e. doses of venom which caused a transient hypotensive response) of venom. P. textilis venom (5-10mug/kg, i.v.) induced cardiovascular collapse in anaesthetized rats, characterized by a rapid decrease in systolic blood pressure until non recordable. Prior administration of priming doses of P. textilis venom (2 and 3mug/kg) or, at least, 4-5 doses of O. scutellatus (2mug/kg, i.v.) or Daboia russelii limitis (20mug/kg, i.v.) venoms prevented cardiovascular collapse induced by P. textilis venom. Moreover, early collapse was also inhibited by prior administration of 2 discrete doses of Acanthophis rugosus venom. Prior administration of commercial polyvalent snake antivenom (500-3000 units/kg, i.v.) or heparin (300 units/kg, i.v.) also inhibited P. textilis venom-induced cardiovascular collapse. Our results indicate that P. textilis venom-induced cardiovascular collapse can be prevented by prior administration of sub lethal doses of venom from P. textilis, O. scutellatus, A. rugosus and D. russelii limitis. This suggests that sudden cardiovascular collapse following envenoming is likely to involve a common mechanism/pathway activated by different snake venoms.
UR - http://www.sciencedirect.com/science/article/pii/S0378427413011077
U2 - 10.1016/j.toxlet.2013.06.235
DO - 10.1016/j.toxlet.2013.06.235
M3 - Article
VL - 221
SP - 205
EP - 211
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 3
ER -