TY - JOUR
T1 - An epidermal microRNA regulates neuronal migration through control of the cellular glycosylation state
AU - Pedersen, Mikael E
AU - Snieckute, Goda
AU - Kagias, Konstantinos
AU - Nehammer, Camilla
AU - Multhaupt, Hinke A B
AU - Couchman, John R
AU - Pocock, Roger D J
PY - 2013
Y1 - 2013
N2 - An appropriate balance in glycosylation of proteoglycans is crucial for their ability to regulate animal development. Here, we report that the Caenorhabditis elegans microRNA mir-79, an ortholog of mammalian miR-9, controls sugar-chain homeostasis by targeting two proteins in the proteoglycan biosynthetic pathway: a chondroitin synthase (SQV-5; squashed vulva-5) and a uridine 5 -diphosphate-sugar transporter (SQV-7). Loss of mir-79 causes neurodevelopmental defects through SQV-5 and SQV-7 dysregulation in the epidermis. This results in a partial shutdown of heparan sulfate biosynthesis that impinges on a LON-2/glypican pathway and disrupts neuronal migration. Our results identify a regulatory axis controlled by a conserved microRNA that maintains proteoglycan homeostasis in cells.
AB - An appropriate balance in glycosylation of proteoglycans is crucial for their ability to regulate animal development. Here, we report that the Caenorhabditis elegans microRNA mir-79, an ortholog of mammalian miR-9, controls sugar-chain homeostasis by targeting two proteins in the proteoglycan biosynthetic pathway: a chondroitin synthase (SQV-5; squashed vulva-5) and a uridine 5 -diphosphate-sugar transporter (SQV-7). Loss of mir-79 causes neurodevelopmental defects through SQV-5 and SQV-7 dysregulation in the epidermis. This results in a partial shutdown of heparan sulfate biosynthesis that impinges on a LON-2/glypican pathway and disrupts neuronal migration. Our results identify a regulatory axis controlled by a conserved microRNA that maintains proteoglycan homeostasis in cells.
UR - http://www.ncbi.nlm.nih.gov/pubmed/24052309
U2 - 10.1126/science.1242528
DO - 10.1126/science.1242528
M3 - Article
VL - 341
SP - 1404
EP - 1408
JO - Science
JF - Science
SN - 0036-8075
IS - 6152
ER -