An electrical impedance-based assay to examine functions of various placental cell types in vitro

Tejasvy Chollangi; Hélène Clabault; Andrée Anne Hudon Thibeault; Hannah E.J. Yong; Shagun Narula; Ellen Menkhorst; J. Thomas Sanderson; Cathy Vaillancourt; Padma Murthi

doi:10.1007/978-1-4939-7498-6_20

An electrical impedance-based assay to examine functions of various placental cell types in vitro

Tejasvy Chollangi, Hélène Clabault, Andrée Anne Hudon Thibeault, Hannah E.J. Yong, Shagun Narula, Ellen Menkhorst, J. Thomas Sanderson, Cathy Vaillancourt, Padma Murthi

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

2 Citations (Scopus)

Abstract

In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.

Original language	English
Title of host publication	Preeclampsia
Subtitle of host publication	Methods and Protocols
Editors	Padma Murthi, Cathy Vaillancourt
Place of Publication	NY USA
Publisher	Humana Press
Pages	267-276
Number of pages	10
Volume	1710
ISBN (Electronic)	9781493974986
ISBN (Print)	9781493974979
DOIs	https://doi.org/10.1007/978-1-4939-7498-6_20
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1710
ISSN (Print)	1064-3745

Keywords

Adhesion
Apoptosis
Functional assays
Invasion
Migration
Proliferation
xCELLigence® RTCA systems

Access to Document

10.1007/978-1-4939-7498-6_20

Cite this

Chollangi, T., Clabault, H., Thibeault, A. A. H., Yong, H. E. J., Narula, S., Menkhorst, E., Sanderson, J. T., Vaillancourt, C., & Murthi, P. (2018). An electrical impedance-based assay to examine functions of various placental cell types in vitro. In P. Murthi, & C. Vaillancourt (Eds.), Preeclampsia: Methods and Protocols (Vol. 1710, pp. 267-276). (Methods in Molecular Biology; Vol. 1710). Humana Press. https://doi.org/10.1007/978-1-4939-7498-6_20

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abstract = "In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.",

keywords = "Adhesion, Apoptosis, Functional assays, Invasion, Migration, Proliferation, xCELLigence{\textregistered} RTCA systems",

author = "Tejasvy Chollangi and H{\'e}l{\`e}ne Clabault and Thibeault, {Andr{\'e}e Anne Hudon} and Yong, {Hannah E.J.} and Shagun Narula and Ellen Menkhorst and Sanderson, {J. Thomas} and Cathy Vaillancourt and Padma Murthi",

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doi = "10.1007/978-1-4939-7498-6_20",

language = "English",

isbn = "9781493974979 ",

volume = "1710",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

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address = "United States of America",

}

Chollangi, T, Clabault, H, Thibeault, AAH, Yong, HEJ, Narula, S, Menkhorst, E, Sanderson, JT, Vaillancourt, C & Murthi, P 2018, An electrical impedance-based assay to examine functions of various placental cell types in vitro. in P Murthi & C Vaillancourt (eds), Preeclampsia: Methods and Protocols. vol. 1710, Methods in Molecular Biology, vol. 1710, Humana Press, NY USA, pp. 267-276. https://doi.org/10.1007/978-1-4939-7498-6_20

An electrical impedance-based assay to examine functions of various placental cell types in vitro. / Chollangi, Tejasvy; Clabault, Hélène; Thibeault, Andrée Anne Hudon et al.
Preeclampsia: Methods and Protocols. ed. / Padma Murthi; Cathy Vaillancourt. Vol. 1710 NY USA: Humana Press, 2018. p. 267-276 (Methods in Molecular Biology; Vol. 1710).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - An electrical impedance-based assay to examine functions of various placental cell types in vitro

AU - Chollangi, Tejasvy

AU - Clabault, Hélène

AU - Thibeault, Andrée Anne Hudon

AU - Yong, Hannah E.J.

AU - Narula, Shagun

AU - Menkhorst, Ellen

AU - Sanderson, J. Thomas

AU - Vaillancourt, Cathy

AU - Murthi, Padma

PY - 2018

Y1 - 2018

N2 - In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.

AB - In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.

KW - Adhesion

KW - Apoptosis

KW - Functional assays

KW - Invasion

KW - Migration

KW - Proliferation

KW - xCELLigence® RTCA systems

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DO - 10.1007/978-1-4939-7498-6_20

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AN - SCOPUS:85037051787

SN - 9781493974979

VL - 1710

T3 - Methods in Molecular Biology

SP - 267

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BT - Preeclampsia

A2 - Murthi, Padma

A2 - Vaillancourt, Cathy

PB - Humana Press

CY - NY USA

ER -

An electrical impedance-based assay to examine functions of various placental cell types in vitro

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