An electrical impedance-based assay to examine functions of various placental cell types in vitro

Tejasvy Chollangi, Hélène Clabault, Andrée Anne Hudon Thibeault, Hannah E.J. Yong, Shagun Narula, Ellen Menkhorst, J. Thomas Sanderson, Cathy Vaillancourt, Padma Murthi

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.

Original languageEnglish
Title of host publicationPreeclampsia
Subtitle of host publicationMethods and Protocols
EditorsPadma Murthi, Cathy Vaillancourt
Place of PublicationNY USA
PublisherHumana Press
Pages267-276
Number of pages10
Volume1710
ISBN (Electronic) 978-1-4939-7498-6
ISBN (Print)978-1-4939-7497-9
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1710
ISSN (Print)1064-3745

Keywords

  • Adhesion
  • Apoptosis
  • Functional assays
  • Invasion
  • Migration
  • Proliferation
  • xCELLigence® RTCA systems

Cite this

Chollangi, T., Clabault, H., Thibeault, A. A. H., Yong, H. E. J., Narula, S., Menkhorst, E., ... Murthi, P. (2018). An electrical impedance-based assay to examine functions of various placental cell types in vitro. In P. Murthi, & C. Vaillancourt (Eds.), Preeclampsia: Methods and Protocols (Vol. 1710, pp. 267-276). (Methods in Molecular Biology; Vol. 1710). NY USA: Humana Press. https://doi.org/10.1007/978-1-4939-7498-6_20
Chollangi, Tejasvy ; Clabault, Hélène ; Thibeault, Andrée Anne Hudon ; Yong, Hannah E.J. ; Narula, Shagun ; Menkhorst, Ellen ; Sanderson, J. Thomas ; Vaillancourt, Cathy ; Murthi, Padma. / An electrical impedance-based assay to examine functions of various placental cell types in vitro. Preeclampsia: Methods and Protocols. editor / Padma Murthi ; Cathy Vaillancourt. Vol. 1710 NY USA : Humana Press, 2018. pp. 267-276 (Methods in Molecular Biology).
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Chollangi, T, Clabault, H, Thibeault, AAH, Yong, HEJ, Narula, S, Menkhorst, E, Sanderson, JT, Vaillancourt, C & Murthi, P 2018, An electrical impedance-based assay to examine functions of various placental cell types in vitro. in P Murthi & C Vaillancourt (eds), Preeclampsia: Methods and Protocols. vol. 1710, Methods in Molecular Biology, vol. 1710, Humana Press, NY USA, pp. 267-276. https://doi.org/10.1007/978-1-4939-7498-6_20

An electrical impedance-based assay to examine functions of various placental cell types in vitro. / Chollangi, Tejasvy; Clabault, Hélène; Thibeault, Andrée Anne Hudon; Yong, Hannah E.J.; Narula, Shagun; Menkhorst, Ellen; Sanderson, J. Thomas; Vaillancourt, Cathy; Murthi, Padma.

Preeclampsia: Methods and Protocols. ed. / Padma Murthi; Cathy Vaillancourt. Vol. 1710 NY USA : Humana Press, 2018. p. 267-276 (Methods in Molecular Biology; Vol. 1710).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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AU - Thibeault, Andrée Anne Hudon

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AU - Narula, Shagun

AU - Menkhorst, Ellen

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AB - In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.

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KW - Functional assays

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KW - Migration

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SN - 978-1-4939-7497-9

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Chollangi T, Clabault H, Thibeault AAH, Yong HEJ, Narula S, Menkhorst E et al. An electrical impedance-based assay to examine functions of various placental cell types in vitro. In Murthi P, Vaillancourt C, editors, Preeclampsia: Methods and Protocols. Vol. 1710. NY USA: Humana Press. 2018. p. 267-276. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7498-6_20