An atypical IgM class platelet cold agglutinin induces GPVI-dependent aggregation of human platelets

Isabel Maria Sanchez Guiu, Irene Martinez-Martinez, Constantino Martinez, Jose Navarro-Fernandez, Faustino Garcia-Candel, Francisca Ferrer-Marin, Vicente Vicente, Steve P Watson, Robert Keith Andrews, Elizabeth Ellen Gardiner, Maria Luisa Lozano, Jose Rivera

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Abstract

Platelet cold agglutinins (PCA) cause pseudothrombocytopenia, spurious thrombocytopenia due to ex vivo platelet clumping, complicating clinical diagnosis, but mechanisms and consequences of PCA are not well defined. Here, we characterised an atypical immunoglobulin (Ig)M PCA in a 37-year-old woman with lifelong bleeding and chronic moderate thrombocytopenia, that induces activation and aggregationof autologous or allogeneic platelets via interaction with platelet glycoprotein (GP)VI. Patient temperature-dependent pseudothrombocytopenia was EDTA-independent, but was prevented by integrin aIIb?3 blockade. Unstimulated patient platelets revealed elevated levels of bound IgM, increased expression of activation markers (P-selectin and CD63), low GPVI levels and abnormally high thromboxane (TX)A2 production. Patient serum induced temperature- and aIIb?3-dependent decrease of platelet count in allogeneic donor citrated platelet-rich plasma (PRP), but not in PRP from Glanzmann?s thrombasthenia or afibrinogenaemia patients. In allogeneic platelets, patient plasma induced shape change, P-selectin and CD63 expression,14C-serotonin release, and TXA2 production. Activation was not inhibited by aspirin, cangrelor or blocking anti-Fc receptor (Fc?RIIA) antibody, but was abrogated by inhibitors of Src and Syk, and by a soluble GPVI-Fc fusion protein. GPVI-deficient platelets were not activated by patient plasma. These data provide the first evidence for an IgM PCA causing platelet activation/aggregation via GPVI. The PCA activity persisted over a five-year follow-up period, supporting a causative role in patient chronic thrombocytopenia and bleeding.
Original languageEnglish
Pages (from-to)313 - 324
Number of pages12
JournalThrombosis and Haemostasis
Volume114
Issue number2
DOIs
Publication statusPublished - 2015

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