An assessment of the stability of MDMA, methamphetamine and THC in oral fluid

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The stability of drugs proscribed under the Victorian Road Safety Act 1986 (MDMA, MA and THC) was determined in authentic drug-positive oral fluid following short-term storage at room temperature, 4?C, fluctuating temperatures and long-term storage at ?20 ?C. Following pH adjustment with ammonium carbonate solution, drugs were extracted using chloroform:isopropanol (9:1) and analysed by LC-MS/MS operating in multiple reaction monitoring mode. The results were analysed using Wilcoxon matched-pairs signed ranks and interpreted in the context of AS4760:2006 cut-offs. For MA and MDMA only minor changes occurred following all storage conditions, but THC concentrations were reduced by 70?87 (n=10) following storage at fluctuating temperatures (room temperature to 60?C) for 2 weeks. Of the 10 samples that were positive for THC prior to storage, only one remained positive after 2 weeks. This has significant implications for the interpretation of THC concentrations in cases where the possibility exists that samples were stored at higher temperatures.
Original languageEnglish
Pages (from-to)397 - 410
Number of pages14
JournalAustralian Journal of Forensic Sciences
Volume46
Issue number4
DOIs
Publication statusPublished - 2014

Cite this

@article{0489f490313d44c0adb07828c4fd1618,
title = "An assessment of the stability of MDMA, methamphetamine and THC in oral fluid",
abstract = "The stability of drugs proscribed under the Victorian Road Safety Act 1986 (MDMA, MA and THC) was determined in authentic drug-positive oral fluid following short-term storage at room temperature, 4?C, fluctuating temperatures and long-term storage at ?20 ?C. Following pH adjustment with ammonium carbonate solution, drugs were extracted using chloroform:isopropanol (9:1) and analysed by LC-MS/MS operating in multiple reaction monitoring mode. The results were analysed using Wilcoxon matched-pairs signed ranks and interpreted in the context of AS4760:2006 cut-offs. For MA and MDMA only minor changes occurred following all storage conditions, but THC concentrations were reduced by 70?87 (n=10) following storage at fluctuating temperatures (room temperature to 60?C) for 2 weeks. Of the 10 samples that were positive for THC prior to storage, only one remained positive after 2 weeks. This has significant implications for the interpretation of THC concentrations in cases where the possibility exists that samples were stored at higher temperatures.",
author = "Turfus, {Sophie C} and Mark Chu and Dimitri Gerostamoulos and Drummer, {Olaf Heino}",
year = "2014",
doi = "10.1080/00450618.2013.879204",
language = "English",
volume = "46",
pages = "397 -- 410",
journal = "Australian Journal of Forensic Sciences",
issn = "0045-0618",
publisher = "Taylor & Francis",
number = "4",

}

An assessment of the stability of MDMA, methamphetamine and THC in oral fluid. / Turfus, Sophie C; Chu, Mark; Gerostamoulos, Dimitri; Drummer, Olaf Heino.

In: Australian Journal of Forensic Sciences, Vol. 46, No. 4, 2014, p. 397 - 410.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - An assessment of the stability of MDMA, methamphetamine and THC in oral fluid

AU - Turfus, Sophie C

AU - Chu, Mark

AU - Gerostamoulos, Dimitri

AU - Drummer, Olaf Heino

PY - 2014

Y1 - 2014

N2 - The stability of drugs proscribed under the Victorian Road Safety Act 1986 (MDMA, MA and THC) was determined in authentic drug-positive oral fluid following short-term storage at room temperature, 4?C, fluctuating temperatures and long-term storage at ?20 ?C. Following pH adjustment with ammonium carbonate solution, drugs were extracted using chloroform:isopropanol (9:1) and analysed by LC-MS/MS operating in multiple reaction monitoring mode. The results were analysed using Wilcoxon matched-pairs signed ranks and interpreted in the context of AS4760:2006 cut-offs. For MA and MDMA only minor changes occurred following all storage conditions, but THC concentrations were reduced by 70?87 (n=10) following storage at fluctuating temperatures (room temperature to 60?C) for 2 weeks. Of the 10 samples that were positive for THC prior to storage, only one remained positive after 2 weeks. This has significant implications for the interpretation of THC concentrations in cases where the possibility exists that samples were stored at higher temperatures.

AB - The stability of drugs proscribed under the Victorian Road Safety Act 1986 (MDMA, MA and THC) was determined in authentic drug-positive oral fluid following short-term storage at room temperature, 4?C, fluctuating temperatures and long-term storage at ?20 ?C. Following pH adjustment with ammonium carbonate solution, drugs were extracted using chloroform:isopropanol (9:1) and analysed by LC-MS/MS operating in multiple reaction monitoring mode. The results were analysed using Wilcoxon matched-pairs signed ranks and interpreted in the context of AS4760:2006 cut-offs. For MA and MDMA only minor changes occurred following all storage conditions, but THC concentrations were reduced by 70?87 (n=10) following storage at fluctuating temperatures (room temperature to 60?C) for 2 weeks. Of the 10 samples that were positive for THC prior to storage, only one remained positive after 2 weeks. This has significant implications for the interpretation of THC concentrations in cases where the possibility exists that samples were stored at higher temperatures.

UR - http://www.tandfonline.com/doi/pdf/10.1080/00450618.2013.879204

U2 - 10.1080/00450618.2013.879204

DO - 10.1080/00450618.2013.879204

M3 - Article

VL - 46

SP - 397

EP - 410

JO - Australian Journal of Forensic Sciences

JF - Australian Journal of Forensic Sciences

SN - 0045-0618

IS - 4

ER -