Abstract
Presented here is a cell-suspension model for positive selection using thymocytes from αβ-TCR (H-2Db-restricted) transgenic mice specific to the lymphocytic choriomeningitis virus (LCMV) on a nonselecting MHC background (H-2(d) or TAP-1 -/-), cocultured with freshly isolated adult thymus stromal cells of the selecting MHC type. The thymic stromal cells alone induced positive selection of functional CD4-CD8+ cells whose kinetics and efficiency were enhanced by nominal peptide. Fibroblasts expressing the selecting MHC alone did not induce positive selection; however, together with nonselecting stroma and nominal peptide, there was inefficient positive. These results suggest multiple signaling in positive selection with selection events able to occur on multiple-cell types. The ease with which this model can be manipulated should greatly facilitate the resolution of the mechanisms of positive selection in normal and pathological states.
Original language | English |
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Pages (from-to) | 157-170 |
Number of pages | 14 |
Journal | Developmental Immunology |
Volume | 6 |
Issue number | 3-4 |
DOIs | |
Publication status | Published - 1 Jan 1998 |
Keywords
- LCMV
- Peptides
- Positive selection
- T-cell differentiation
- Thymic selection