Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

Muamer Dervisevic; Emre Çevik; Mehmet Şenel; Cevdet Nergiz; M. Fatih Abasiyanik

doi:10.1016/j.jelechem.2016.06.033

Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

Muamer Dervisevic, Emre Çevik, Mehmet Şenel, Cevdet Nergiz, M. Fatih Abasiyanik

Research output: Contribution to journal › Article › Research › peer-review

50 Citations (Scopus)

Abstract

An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.

Original language	English
Pages (from-to)	18-24
Number of pages	7
Journal	Journal of Electroanalytical Chemistry
Volume	776
DOIs	https://doi.org/10.1016/j.jelechem.2016.06.033
Publication status	Published - 1 Sept 2016
Externally published	Yes

Keywords

Amperometric biosensor
Boronic acid
Cholesterol
Cholesterol oxidase
Electropolymerization
Reconstitution

Access to Document

10.1016/j.jelechem.2016.06.033

Cite this

@article{5c4281f4e0b447ecbc77ace199d50b96,

title = "Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers",

abstract = "An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.",

keywords = "Amperometric biosensor, Boronic acid, Cholesterol, Cholesterol oxidase, Electropolymerization, Reconstitution",

author = "Muamer Dervisevic and Emre {\c C}evik and Mehmet {\c S}enel and Cevdet Nergiz and Abasiyanik, {M. Fatih}",

year = "2016",

month = sep,

day = "1",

doi = "10.1016/j.jelechem.2016.06.033",

language = "English",

volume = "776",

pages = "18--24",

journal = "Journal of Electroanalytical Chemistry",

issn = "1572-6657",

publisher = "Elsevier",

}

TY - JOUR

T1 - Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

AU - Dervisevic, Muamer

AU - Çevik, Emre

AU - Şenel, Mehmet

AU - Nergiz, Cevdet

AU - Abasiyanik, M. Fatih

PY - 2016/9/1

Y1 - 2016/9/1

N2 - An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.

AB - An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.

KW - Amperometric biosensor

KW - Boronic acid

KW - Cholesterol

KW - Cholesterol oxidase

KW - Electropolymerization

KW - Reconstitution

UR - http://www.scopus.com/inward/record.url?scp=84977071243&partnerID=8YFLogxK

U2 - 10.1016/j.jelechem.2016.06.033

DO - 10.1016/j.jelechem.2016.06.033

M3 - Article

AN - SCOPUS:84977071243

SN - 1572-6657

VL - 776

SP - 18

EP - 24

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

ER -

Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

Abstract

Keywords

Access to Document

Other files and links

Cite this