Abstract
An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.
Original language | English |
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Pages (from-to) | 18-24 |
Number of pages | 7 |
Journal | Journal of Electroanalytical Chemistry |
Volume | 776 |
DOIs | |
Publication status | Published - 1 Sept 2016 |
Externally published | Yes |
Keywords
- Amperometric biosensor
- Boronic acid
- Cholesterol
- Cholesterol oxidase
- Electropolymerization
- Reconstitution