Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers

Muamer Dervisevic, Emre Çevik, Mehmet Şenel, Cevdet Nergiz, M. Fatih Abasiyanik

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25 Citations (Scopus)

Abstract

An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinucleotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiophene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic acid. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalJournal of Electroanalytical Chemistry
Volume776
DOIs
Publication statusPublished - 1 Sep 2016
Externally publishedYes

Keywords

  • Amperometric biosensor
  • Boronic acid
  • Cholesterol
  • Cholesterol oxidase
  • Electropolymerization
  • Reconstitution

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