Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis

Yu Liu, Vijesh Vaghjiani, Jing Tee, Kelly To, Peng Cui, Ding Oh, Ursula Manuelpillai, Ban-Hock Toh, Moh Chan

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73 Citations (Scopus)

Abstract

Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-beta (TGF-beta) for immunosuppression. Neutralization of TGF-beta or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.
Original languageEnglish
Article numbere35758
Number of pages9
JournalPLoS ONE
Volume7
Issue number4
DOIs
Publication statusPublished - 2012

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