Abstract
| Original language | English |
|---|---|
| Title of host publication | Current Protocols in Stem Cell Biology (Unit 1E.6) |
| Editors | Andrew Elfanty, Richard Gregory, Roger Patient, Thorsten Schlager, Evan Y Snyder, Yukiko Yamashita |
| Place of Publication | United States |
| Publisher | John Wiley & Sons |
| Pages | 1 - 25 |
| Number of pages | 25 |
| ISBN (Print) | 1941-7322 |
| DOIs | |
| Publication status | Published - 2010 |
Cite this
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Amnion epithelial cell isolation and characterization for clinical use. / Murphy, Sean; Rosli, Sharina; Acharya, Rutu; Mathias, Louisa; Lim, Rebecca; Wallace, Euan; Jenkin, Graham.
Current Protocols in Stem Cell Biology (Unit 1E.6). ed. / Andrew Elfanty; Richard Gregory; Roger Patient; Thorsten Schlager; Evan Y Snyder; Yukiko Yamashita. United States : John Wiley & Sons, 2010. p. 1 - 25.Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review
TY - CHAP
T1 - Amnion epithelial cell isolation and characterization for clinical use
AU - Murphy, Sean
AU - Rosli, Sharina
AU - Acharya, Rutu
AU - Mathias, Louisa
AU - Lim, Rebecca
AU - Wallace, Euan
AU - Jenkin, Graham
PY - 2010
Y1 - 2010
N2 - Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation, culture, and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype, cell cycle distribution, and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR, immunocytochemistry, and histology.
AB - Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation, culture, and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype, cell cycle distribution, and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR, immunocytochemistry, and histology.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20373516
U2 - 10.1002/9780470151808.sc01e06s13
DO - 10.1002/9780470151808.sc01e06s13
M3 - Chapter (Book)
SN - 1941-7322
SP - 1
EP - 25
BT - Current Protocols in Stem Cell Biology (Unit 1E.6)
A2 - Elfanty, Andrew
A2 - Gregory, Richard
A2 - Patient, Roger
A2 - Schlager, Thorsten
A2 - Snyder, Evan Y
A2 - Yamashita, Yukiko
PB - John Wiley & Sons
CY - United States
ER -