Amnion epithelial cell isolation and characterization for clinical use

Sean Murphy, Sharina Rosli, Rutu Acharya, Louisa Mathias, Rebecca Lim, Euan Wallace, Graham Jenkin

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation, culture, and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype, cell cycle distribution, and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR, immunocytochemistry, and histology.
Original languageEnglish
Title of host publicationCurrent Protocols in Stem Cell Biology (Unit 1E.6)
EditorsAndrew Elfanty, Richard Gregory, Roger Patient, Thorsten Schlager, Evan Y Snyder, Yukiko Yamashita
Place of PublicationUnited States
PublisherJohn Wiley & Sons
Pages1 - 25
Number of pages25
ISBN (Print)1941-7322
DOIs
Publication statusPublished - 2010

Cite this

Murphy, S., Rosli, S., Acharya, R., Mathias, L., Lim, R., Wallace, E., & Jenkin, G. (2010). Amnion epithelial cell isolation and characterization for clinical use. In A. Elfanty, R. Gregory, R. Patient, T. Schlager, E. Y. Snyder, & Y. Yamashita (Eds.), Current Protocols in Stem Cell Biology (Unit 1E.6) (pp. 1 - 25). United States: John Wiley & Sons. https://doi.org/10.1002/9780470151808.sc01e06s13
Murphy, Sean ; Rosli, Sharina ; Acharya, Rutu ; Mathias, Louisa ; Lim, Rebecca ; Wallace, Euan ; Jenkin, Graham. / Amnion epithelial cell isolation and characterization for clinical use. Current Protocols in Stem Cell Biology (Unit 1E.6). editor / Andrew Elfanty ; Richard Gregory ; Roger Patient ; Thorsten Schlager ; Evan Y Snyder ; Yukiko Yamashita. United States : John Wiley & Sons, 2010. pp. 1 - 25
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Murphy, S, Rosli, S, Acharya, R, Mathias, L, Lim, R, Wallace, E & Jenkin, G 2010, Amnion epithelial cell isolation and characterization for clinical use. in A Elfanty, R Gregory, R Patient, T Schlager, EY Snyder & Y Yamashita (eds), Current Protocols in Stem Cell Biology (Unit 1E.6). John Wiley & Sons, United States, pp. 1 - 25. https://doi.org/10.1002/9780470151808.sc01e06s13

Amnion epithelial cell isolation and characterization for clinical use. / Murphy, Sean; Rosli, Sharina; Acharya, Rutu; Mathias, Louisa; Lim, Rebecca; Wallace, Euan; Jenkin, Graham.

Current Protocols in Stem Cell Biology (Unit 1E.6). ed. / Andrew Elfanty; Richard Gregory; Roger Patient; Thorsten Schlager; Evan Y Snyder; Yukiko Yamashita. United States : John Wiley & Sons, 2010. p. 1 - 25.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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Murphy S, Rosli S, Acharya R, Mathias L, Lim R, Wallace E et al. Amnion epithelial cell isolation and characterization for clinical use. In Elfanty A, Gregory R, Patient R, Schlager T, Snyder EY, Yamashita Y, editors, Current Protocols in Stem Cell Biology (Unit 1E.6). United States: John Wiley & Sons. 2010. p. 1 - 25 https://doi.org/10.1002/9780470151808.sc01e06s13