Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation, culture, and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype, cell cycle distribution, and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR, immunocytochemistry, and histology.
|Title of host publication||Current Protocols in Stem Cell Biology (Unit 1E.6)|
|Editors||Andrew Elfanty, Richard Gregory, Roger Patient, Thorsten Schlager, Evan Y Snyder, Yukiko Yamashita|
|Place of Publication||United States|
|Publisher||John Wiley & Sons|
|Pages||1 - 25|
|Number of pages||25|
|Publication status||Published - 2010|