Amnion epithelial cell isolation and characterization for clinical use

Sean Murphy, Sharina Rosli, Rutu Acharya, Louisa Mathias, Rebecca Lim, Euan Wallace, Graham Jenkin

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

117 Citations (Scopus)

Abstract

Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into cells of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence, hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation, culture, and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype, cell cycle distribution, and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR, immunocytochemistry, and histology.
Original languageEnglish
Title of host publicationCurrent Protocols in Stem Cell Biology (Unit 1E.6)
EditorsAndrew Elfanty, Richard Gregory, Roger Patient, Thorsten Schlager, Evan Y Snyder, Yukiko Yamashita
Place of PublicationUnited States
PublisherJohn Wiley & Sons
Pages1 - 25
Number of pages25
ISBN (Print)1941-7322
DOIs
Publication statusPublished - 2010

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