TY - JOUR
T1 - Amelioration of hyperglycemia-induced oxidative damage in ARPE-19 cells by myricetin derivatives isolated from Syzygium malaccense
AU - Arumugam, Bavani
AU - Palanisamy, Uma Devi
AU - Chua, Kek Heng
AU - Kuppusamy, Umah Rani
N1 - Funding Information:
This study was financially supported by the Ministry of Education Malaysia under High Impact Research MoE Grant (UM.C/HIR/MoE/MED/11 E000042-20001); and University of Malaya, Kuala Lumpur, Malaysia under Postgraduate Research Grant ( PG018/2012B ).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/4
Y1 - 2020/4
N2 - Myricetin derivatives (F2) isolated from leaf extract of Syzygium malaccense (Malay apple) which contains myricitrin predominantly, could potentially serve as functional food ingredient based on previous findings. The present study aimed to investigate the protective effects of F2 against hyperglycemia-induced oxidative stress in ARPE-19 (retinal pigment epithelium) cells; a diabetic retinopathy (DR) model. F2 showed effective inhibition of advanced glycation end products (AGE) formation. The derivatives attenuated high glucose (30 mM)-induced stress condition in ARPE-19 cells by reducing intracellular reactive oxygen species (ROS). At transcriptional level, F2 activated Nrf2 pathway besides upregulating antioxidant enzymes and other protective factors. In addition, protective effect of F2 against formation of AGE correlated with its ability to downregulate gene expression of inflammatory factor, NFkB1 and receptor for AGE. The findings showed that F2 was effective in preventing oxidative damage-induced by high glucose in ARPE-19 and could be developed as an adjuvant to manage DR.
AB - Myricetin derivatives (F2) isolated from leaf extract of Syzygium malaccense (Malay apple) which contains myricitrin predominantly, could potentially serve as functional food ingredient based on previous findings. The present study aimed to investigate the protective effects of F2 against hyperglycemia-induced oxidative stress in ARPE-19 (retinal pigment epithelium) cells; a diabetic retinopathy (DR) model. F2 showed effective inhibition of advanced glycation end products (AGE) formation. The derivatives attenuated high glucose (30 mM)-induced stress condition in ARPE-19 cells by reducing intracellular reactive oxygen species (ROS). At transcriptional level, F2 activated Nrf2 pathway besides upregulating antioxidant enzymes and other protective factors. In addition, protective effect of F2 against formation of AGE correlated with its ability to downregulate gene expression of inflammatory factor, NFkB1 and receptor for AGE. The findings showed that F2 was effective in preventing oxidative damage-induced by high glucose in ARPE-19 and could be developed as an adjuvant to manage DR.
KW - Antiglycation
KW - Diabetic retinopathy
KW - Hyperglycemia
KW - Myricetin (PubChem CID: 5281672)
KW - Myricetin 3-alpha-L-arabinofuranoside (PubChem CID: 14524431)
KW - Myricetin 3′-glucoside (PubChem CID: 44259426)
KW - Myricitrin
KW - Myricitrin (PubChem CID: 5281673)
KW - Oxidative stress
KW - Quercetin (PubChem CID: 5280343)
KW - Syzygium malaccense
UR - http://www.scopus.com/inward/record.url?scp=85079514980&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2020.103844
DO - 10.1016/j.jff.2020.103844
M3 - Article
AN - SCOPUS:85079514980
VL - 67
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
M1 - 103844
ER -
