Alternative splicing generates two isoforms of the β3-adrenoceptor which are differentially expressed in mouse tissues

B. A. Evans, M. Papaioannou, S. Hamilton, R. J. Summers

Research output: Contribution to journalArticleResearchpeer-review

Abstract

1. The β3-adrenoceptor (AR) differs from the β1-AR and β2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3-AR which differ within the coding region. 2. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse β3-adrenoceptor. The novel β(3b)-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β(3a)-AR (RFDGYEGARPFPT). 3. β(3b)-AR mRNA is differentially expressed in mouse tissues, with levels relative to β(3a)-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.

Original languageEnglish
Pages (from-to)1525-1531
Number of pages7
JournalBritish Journal of Pharmacology
Volume127
Issue number6
DOIs
Publication statusPublished - 10 Aug 1999

Keywords

  • β-adrenoceptor
  • Adipose tissue
  • Brain
  • Ileum
  • Mouse
  • Splice variants

Cite this

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title = "Alternative splicing generates two isoforms of the β3-adrenoceptor which are differentially expressed in mouse tissues",
abstract = "1. The β3-adrenoceptor (AR) differs from the β1-AR and β2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3-AR which differ within the coding region. 2. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse β3-adrenoceptor. The novel β(3b)-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β(3a)-AR (RFDGYEGARPFPT). 3. β(3b)-AR mRNA is differentially expressed in mouse tissues, with levels relative to β(3a)-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.",
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Alternative splicing generates two isoforms of the β3-adrenoceptor which are differentially expressed in mouse tissues. / Evans, B. A.; Papaioannou, M.; Hamilton, S.; Summers, R. J.

In: British Journal of Pharmacology, Vol. 127, No. 6, 10.08.1999, p. 1525-1531.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Alternative splicing generates two isoforms of the β3-adrenoceptor which are differentially expressed in mouse tissues

AU - Evans, B. A.

AU - Papaioannou, M.

AU - Hamilton, S.

AU - Summers, R. J.

PY - 1999/8/10

Y1 - 1999/8/10

N2 - 1. The β3-adrenoceptor (AR) differs from the β1-AR and β2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3-AR which differ within the coding region. 2. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse β3-adrenoceptor. The novel β(3b)-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β(3a)-AR (RFDGYEGARPFPT). 3. β(3b)-AR mRNA is differentially expressed in mouse tissues, with levels relative to β(3a)-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.

AB - 1. The β3-adrenoceptor (AR) differs from the β1-AR and β2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3-AR which differ within the coding region. 2. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse β3-adrenoceptor. The novel β(3b)-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β(3a)-AR (RFDGYEGARPFPT). 3. β(3b)-AR mRNA is differentially expressed in mouse tissues, with levels relative to β(3a)-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.

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KW - Adipose tissue

KW - Brain

KW - Ileum

KW - Mouse

KW - Splice variants

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