Abstract
Regulation of organ growth is a poorly understood process. In the long bones, the growth plates (GPs) drive elongation by generating a scaffold progressively replaced by bone. Although studies have focused on intrinsic GP regulation, classic and recent experiments suggest that local signals also modulate GP function. We devised a genetic mouse model to study extrinsic long bone growth modulation, in which injury is specifically induced in the left hindlimb, such that the right hindlimb serves as an internal control. Remarkably, when only mesenchyme cells surrounding postnatal GPs were killed, left bone growth was nevertheless reduced. GP signaling was impaired by altered paracrine signals from the knee joint, including activation of the injury response and, in neonates, dampened IGF1 production. Importantly, only the combined prevention of both responses rescued neonatal growth. Thus, we identified signals from the knee joint that modulate bone growth and could underlie establishment of body proportions.
| Original language | English |
|---|---|
| Article number | e27210 |
| Number of pages | 24 |
| Journal | eLife |
| Volume | 6 |
| DOIs | |
| Publication status | Published - 25 Jul 2017 |
| Externally published | Yes |
Cite this
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Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth. / Rosello-Diez, Alberto; Stephen, Daniel; Joyner, Alexandra L.
In: eLife, Vol. 6, e27210, 25.07.2017.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth
AU - Rosello-Diez, Alberto
AU - Stephen, Daniel
AU - Joyner, Alexandra L.
PY - 2017/7/25
Y1 - 2017/7/25
N2 - Regulation of organ growth is a poorly understood process. In the long bones, the growth plates (GPs) drive elongation by generating a scaffold progressively replaced by bone. Although studies have focused on intrinsic GP regulation, classic and recent experiments suggest that local signals also modulate GP function. We devised a genetic mouse model to study extrinsic long bone growth modulation, in which injury is specifically induced in the left hindlimb, such that the right hindlimb serves as an internal control. Remarkably, when only mesenchyme cells surrounding postnatal GPs were killed, left bone growth was nevertheless reduced. GP signaling was impaired by altered paracrine signals from the knee joint, including activation of the injury response and, in neonates, dampened IGF1 production. Importantly, only the combined prevention of both responses rescued neonatal growth. Thus, we identified signals from the knee joint that modulate bone growth and could underlie establishment of body proportions.
AB - Regulation of organ growth is a poorly understood process. In the long bones, the growth plates (GPs) drive elongation by generating a scaffold progressively replaced by bone. Although studies have focused on intrinsic GP regulation, classic and recent experiments suggest that local signals also modulate GP function. We devised a genetic mouse model to study extrinsic long bone growth modulation, in which injury is specifically induced in the left hindlimb, such that the right hindlimb serves as an internal control. Remarkably, when only mesenchyme cells surrounding postnatal GPs were killed, left bone growth was nevertheless reduced. GP signaling was impaired by altered paracrine signals from the knee joint, including activation of the injury response and, in neonates, dampened IGF1 production. Importantly, only the combined prevention of both responses rescued neonatal growth. Thus, we identified signals from the knee joint that modulate bone growth and could underlie establishment of body proportions.
UR - http://www.scopus.com/inward/record.url?scp=85026778226&partnerID=8YFLogxK
U2 - 10.7554/eLife.27210
DO - 10.7554/eLife.27210
M3 - Article
VL - 6
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e27210
ER -