Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

Meagan A. Cauble, Edward Rothman, Kathleen Welch, Ming Fang, Le T. Duong, Brenda L. Pennypacker, Bradford G. Orr, Mark M. Banaszak Holl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n=11), ovariectomized (OVX; n=12), OVX+200 μg kg−1 alendronate (ALN), 3 × a week for 27 weeks (n=12) and OVX+10 mg kg−1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n=12), OVX (n=12) or OVX+0.05 mg kg−1 17β-estradiol (ERT) 3 × a week for 13 weeks (n=12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52% and 53%, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35% in both cases. ALN and ERT drug treatments reduced the change from 18 to 12%, whereas CatKI treatment reduced the change to 5%.
Original languageEnglish
Article number697
Number of pages4
JournalBoneKEy Reports
Volume4
DOIs
Publication statusPublished - 2015
Externally publishedYes

Cite this

Cauble, Meagan A. ; Rothman, Edward ; Welch, Kathleen ; Fang, Ming ; Duong, Le T. ; Pennypacker, Brenda L. ; Orr, Bradford G. ; Banaszak Holl, Mark M. / Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment. In: BoneKEy Reports. 2015 ; Vol. 4.
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title = "Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment",
abstract = "Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n=11), ovariectomized (OVX; n=12), OVX+200 μg kg−1 alendronate (ALN), 3 × a week for 27 weeks (n=12) and OVX+10 mg kg−1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n=12), OVX (n=12) or OVX+0.05 mg kg−1 17β-estradiol (ERT) 3 × a week for 13 weeks (n=12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52{\%} and 53{\%}, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35{\%} in both cases. ALN and ERT drug treatments reduced the change from 18 to 12{\%}, whereas CatKI treatment reduced the change to 5{\%}.",
author = "Cauble, {Meagan A.} and Edward Rothman and Kathleen Welch and Ming Fang and Duong, {Le T.} and Pennypacker, {Brenda L.} and Orr, {Bradford G.} and {Banaszak Holl}, {Mark M.}",
year = "2015",
doi = "10.1038/bonekey.2015.66",
language = "English",
volume = "4",
journal = "BoneKEy Reports",
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Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment. / Cauble, Meagan A.; Rothman, Edward ; Welch, Kathleen ; Fang, Ming; Duong, Le T.; Pennypacker, Brenda L.; Orr, Bradford G.; Banaszak Holl, Mark M.

In: BoneKEy Reports, Vol. 4, 697, 2015.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

AU - Cauble, Meagan A.

AU - Rothman, Edward

AU - Welch, Kathleen

AU - Fang, Ming

AU - Duong, Le T.

AU - Pennypacker, Brenda L.

AU - Orr, Bradford G.

AU - Banaszak Holl, Mark M.

PY - 2015

Y1 - 2015

N2 - Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n=11), ovariectomized (OVX; n=12), OVX+200 μg kg−1 alendronate (ALN), 3 × a week for 27 weeks (n=12) and OVX+10 mg kg−1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n=12), OVX (n=12) or OVX+0.05 mg kg−1 17β-estradiol (ERT) 3 × a week for 13 weeks (n=12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52% and 53%, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35% in both cases. ALN and ERT drug treatments reduced the change from 18 to 12%, whereas CatKI treatment reduced the change to 5%.

AB - Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n=11), ovariectomized (OVX; n=12), OVX+200 μg kg−1 alendronate (ALN), 3 × a week for 27 weeks (n=12) and OVX+10 mg kg−1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n=12), OVX (n=12) or OVX+0.05 mg kg−1 17β-estradiol (ERT) 3 × a week for 13 weeks (n=12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52% and 53%, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35% in both cases. ALN and ERT drug treatments reduced the change from 18 to 12%, whereas CatKI treatment reduced the change to 5%.

U2 - 10.1038/bonekey.2015.66

DO - 10.1038/bonekey.2015.66

M3 - Article

VL - 4

JO - BoneKEy Reports

JF - BoneKEy Reports

SN - 2047-6396

M1 - 697

ER -