Abstract
Dendritic cells (DCs) have shown promise for use in cancer vaccine and cancer immunotherapy studies. However, we demonstrate that cancer cell lines can negatively interfere with DC generation in granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived cultures, although cancer cells are able to enhance CD80 cell surface activation marker and cytokine secretion. Furthermore, in the presence of cancer cells, GM-CSF-derived DCs are unable to stimulate T-cells. Additional stimulation with toll-like receptor 4 cannot fully reverse the suppressive effect of cancer cells or supernatant. Hence, it is imperative to understand the immunosuppressive effects of cancer on DCs in order for DC-based cancer immunotherapy to be successful.
| Original language | English |
|---|---|
| Pages (from-to) | 1101-1111 |
| Number of pages | 11 |
| Journal | Acta Biochimica et Biophysica Sinica |
| Volume | 48 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2016 |
Keywords
- Cytokines
- Dendritic cells
- Early dendritic cells
- GM-CSF
Cite this
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Alteration of early dendritic cell activation by cancer cell lines predisposes immunosuppression, which cannot be reversed by TLR4 stimulation. / Kong, Ying Ying; Fuchsberger, Martina; Plebanski, Magdalena; Apostolopoulos, Vasso.
In: Acta Biochimica et Biophysica Sinica, Vol. 48, No. 12, 2016, p. 1101-1111.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Alteration of early dendritic cell activation by cancer cell lines predisposes immunosuppression, which cannot be reversed by TLR4 stimulation
AU - Kong, Ying Ying
AU - Fuchsberger, Martina
AU - Plebanski, Magdalena
AU - Apostolopoulos, Vasso
PY - 2016
Y1 - 2016
N2 - Dendritic cells (DCs) have shown promise for use in cancer vaccine and cancer immunotherapy studies. However, we demonstrate that cancer cell lines can negatively interfere with DC generation in granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived cultures, although cancer cells are able to enhance CD80 cell surface activation marker and cytokine secretion. Furthermore, in the presence of cancer cells, GM-CSF-derived DCs are unable to stimulate T-cells. Additional stimulation with toll-like receptor 4 cannot fully reverse the suppressive effect of cancer cells or supernatant. Hence, it is imperative to understand the immunosuppressive effects of cancer on DCs in order for DC-based cancer immunotherapy to be successful.
AB - Dendritic cells (DCs) have shown promise for use in cancer vaccine and cancer immunotherapy studies. However, we demonstrate that cancer cell lines can negatively interfere with DC generation in granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived cultures, although cancer cells are able to enhance CD80 cell surface activation marker and cytokine secretion. Furthermore, in the presence of cancer cells, GM-CSF-derived DCs are unable to stimulate T-cells. Additional stimulation with toll-like receptor 4 cannot fully reverse the suppressive effect of cancer cells or supernatant. Hence, it is imperative to understand the immunosuppressive effects of cancer on DCs in order for DC-based cancer immunotherapy to be successful.
KW - Cytokines
KW - Dendritic cells
KW - Early dendritic cells
KW - GM-CSF
UR - http://www.scopus.com/inward/record.url?scp=85015914667&partnerID=8YFLogxK
U2 - 10.1093/abbs/gmw102
DO - 10.1093/abbs/gmw102
M3 - Article
VL - 48
SP - 1101
EP - 1111
JO - Acta Biochimica et Biophysica Sinica
JF - Acta Biochimica et Biophysica Sinica
SN - 0582-9879
IS - 12
ER -