Abstract
Dendritic cells (DCs) have shown promise for use in cancer vaccine and cancer immunotherapy studies. However, we demonstrate that cancer cell lines can negatively interfere with DC generation in granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived cultures, although cancer cells are able to enhance CD80 cell surface activation marker and cytokine secretion. Furthermore, in the presence of cancer cells, GM-CSF-derived DCs are unable to stimulate T-cells. Additional stimulation with toll-like receptor 4 cannot fully reverse the suppressive effect of cancer cells or supernatant. Hence, it is imperative to understand the immunosuppressive effects of cancer on DCs in order for DC-based cancer immunotherapy to be successful.
Original language | English |
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Pages (from-to) | 1101-1111 |
Number of pages | 11 |
Journal | Acta Biochimica et Biophysica Sinica |
Volume | 48 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- Cytokines
- Dendritic cells
- Early dendritic cells
- GM-CSF
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